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WNT16 elisa kit :: Mouse wingless-type MMTV integration site family, member 16 ELISA Kit

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Catalog # MBS935746
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

wingless-type MMTV integration site family, member 16 (WNT16), ELISA Kit

Popular Item
Also Known As

Mouse Protein Wnt-16, WNT16 ELISA Kit

Product Synonym Names
Mouse Protein Wnt-16 (WNT16) ELISA kit; wingless-type MMTV integration site family; member 16
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS935746 Testing Data
Sequence Length
364
3D Structure
ModBase 3D Structure for Q9QYS1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse WNT16. No significant cross-reactivity or interference between mouse WNT16 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
62.5 pg/ml -4000 pg/ml.
Sensitivity
The minimum detectable dose of mouse WNT16 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WNT16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWNT16purchase
MBS935746 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 16 (WNT16) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT16. The ELISA analytical biochemical technique of the MBS935746 kit is based on WNT16 antibody-WNT16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WNT16 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of WNT16 elisa kit
WNT16 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS935746. Click to request current manual
NCBI/Uniprot data below describe general gene information for WNT16. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,727 Da
NCBI Official Full Name
protein Wnt-16
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 16
NCBI Official Symbol
Wnt16  [Similar Products]
NCBI Official Synonym Symbols
E130309I19Rik
  [Similar Products]
NCBI Protein Information
protein Wnt-16; wingless-related MMTV integration site 16
UniProt Protein Name
Protein Wnt-16
Protein Family
UniProt Gene Name
Wnt16  [Similar Products]
UniProt Entry Name
WNT16_MOUSE
UniProt Comments for WNT16
WNT16: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: frizzled binding; receptor binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; keratinocyte differentiation; neuron differentiation; Wnt receptor signaling pathway; cell fate commitment; multicellular organismal development; positive regulation of JNK cascade; keratinocyte proliferation; bone remodeling
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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