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WNT4 elisa kit :: Human wingless-type MMTV integration site family, member 4 ELISA Kit

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Catalog # MBS934091
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

wingless-type MMTV integration site family, member 4 (WNT4), ELISA Kit

Popular Item
Also Known As

Human Protein Wnt-4, WNT4 ELISA Kit

Product Synonym Names
Human Protein Wnt-4 (WNT4) ELISA kit; RP1-224A6.7; SERKAL; WNT-4; WNT-4 protein; wingless-type MMTV integration site family; member 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS934091 Testing Data
Sequence Length
351
OMIM
gene 611812
3D Structure
ModBase 3D Structure for P56705
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human WNT4. No significant cross-reactivity or interference between human WNT4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
The minimum detectable dose of human WNT4 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WNT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWNT4purchase
MBS934091 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 4 (WNT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT4. The ELISA analytical biochemical technique of the MBS934091 kit is based on WNT4 antibody-WNT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WNT4 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of WNT4 elisa kit
WNT4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS934091. Click to request current manual
NCBI/Uniprot data below describe general gene information for WNT4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,052 Da
NCBI Official Full Name
protein Wnt-4
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 4
NCBI Official Symbol
NCBI Official Synonym Symbols
WNT-4; SERKAL
  [Similar Products]
NCBI Protein Information
protein Wnt-4
UniProt Protein Name
Protein Wnt-4
UniProt Gene Name
UniProt Synonym Gene Names
UNQ426/PRO864  [Similar Products]
UniProt Entry Name
WNT4_HUMAN
NCBI Summary for WNT4
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for WNT4
WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.23-p35.1

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; Golgi lumen; endoplasmic reticulum lumen; cytoplasm; extracellular region; plasma membrane

Molecular Function: frizzled binding; receptor agonist activity; transcription corepressor activity

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; thyroid stimulating hormone secreting cell differentiation; androgen biosynthetic process; Wnt receptor signaling pathway through beta-catenin; somatotropin secreting cell differentiation; neuron differentiation; regulation of cell-cell adhesion; positive regulation of focal adhesion formation; positive regulation of aldosterone biosynthetic process; positive regulation of stress fiber formation; embryonic epithelial tube formation; kidney development; negative regulation of cell migration; female gonad development; cell fate commitment; positive regulation of meiosis; adrenal gland development; male gonad development; immature T cell proliferation in the thymus; cellular response to starvation; positive regulation of bone mineralization; liver development; positive regulation of osteoblast differentiation; negative regulation of cell differentiation; protein palmitoylation; ureteric bud branching; epithelial to mesenchymal transition; smooth muscle cell differentiation; female sex determination; negative regulation of transcription, DNA-dependent; oocyte development

Disease: Mullerian Aplasia And Hyperandrogenism; 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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