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anti-WNT5A antibody :: Mouse anti-Human WNT5A Antibody

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Catalog # MBS850867
Unit / Price
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  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

WNT5A, Antibody

Popular Item
Full Product Name

WNT5A Antibody

Product Synonym Names
hWNT5A
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
164975
3D Structure
ModBase 3D Structure for P41221
Isotype
IgG1
Clone Number
6F2
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Immunogen
Purified recombinant fragment of WNT5A expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Other Notes
Small volumes of anti-WNT5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-WNT5A antibody
WNT5A: wingless-type MMTV integration site family, member 5A. Entrez Protein: NP_003383. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
Applications Tested/Suitable for anti-WNT5A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-WNT5A antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-WNT5A antibody
Figure 1: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2). Figure 1: Western blot Figure 1:
anti-WNT5A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-WNT5A antibody
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A mouse mAb with DAB staining.
anti-WNT5A antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-WNT5A antibody
Figure 3: Confocal immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
anti-WNT5A antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for WNT5A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42.3kDa
NCBI Official Full Name
protein Wnt-5a isoform 2
NCBI Official Synonym Full Names
Wnt family member 5A
NCBI Official Symbol
WNT5A  [Similar Products]
NCBI Official Synonym Symbols
hWNT5A
  [Similar Products]
NCBI Protein Information
protein Wnt-5a
UniProt Protein Name
Protein Wnt-5a
Protein Family
UniProt Gene Name
WNT5A  [Similar Products]
NCBI Summary for WNT5A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Comments for WNT5A
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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