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WNT5A elisa kit :: Human Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit

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Catalog # MBS2025493 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $290 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $745 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,020 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,450 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), ELISA Kit

Popular Item
Also Known As

Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
380
OMIM
164975
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5A (WNT5A).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WNT5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWNT5Apurchase
MBS2025493 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT5A. The ELISA analytical biochemical technique of the MBS2025493 kit is based on WNT5A antibody-WNT5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WNT5A elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for WNT5A elisa kit

Typical Testing Data/Standard Curve of WNT5A elisa kit
WNT5A elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS2025493. Click to request current manual
NCBI/Uniprot data below describe general gene information for WNT5A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,887 Da
NCBI Official Full Name
Wingless-type MMTV integration site family, member 5A
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 5A
NCBI Official Symbol
WNT5A  [Similar Products]
NCBI Official Synonym Symbols
hWNT5A
  [Similar Products]
NCBI Protein Information
protein Wnt-5a
UniProt Protein Name
Protein Wnt-5a
Protein Family
UniProt Gene Name
WNT5A  [Similar Products]
UniProt Entry Name
WNT5A_HUMAN
NCBI Summary for WNT5A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Comments for WNT5A
WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21-p14

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; receptor tyrosine kinase-like orphan receptor binding; frizzled binding; cytokine activity; receptor agonist activity; transcription factor activity; frizzled-2 binding

Biological Process: activation of MAPK activity; positive regulation of transcription, DNA-dependent; embryonic skeletal development; negative chemotaxis; positive regulation of interleukin-1 beta secretion; negative regulation of synaptogenesis; Wnt receptor signaling pathway through beta-catenin; uterus development; protein amino acid phosphorylation; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; neuron differentiation; positive regulation of fibroblast proliferation; determination of anterior/posterior axis, embryo; positive regulation of mesenchymal cell proliferation; positive regulation of macrophage activation; somitogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; positive regulation of interleukin-6 production; vagina development; negative regulation of fat cell differentiation; keratinocyte differentiation; genitalia development; positive regulation of angiogenesis; Wnt receptor signaling pathway, calcium modulating pathway; positive regulation of protein catabolic process; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; lens development in camera-type eye; axon guidance; wound healing; negative regulation of fibroblast growth factor receptor signaling pathway; hypophysis morphogenesis; positive regulation of cytokine secretion during immune response; palate development; negative regulation of axon extension involved in axon guidance; activation of JNK activity; response to organic substance; establishment of planar polarity; heart looping; negative regulation of epithelial cell proliferation; cervix development; Wnt receptor signaling pathway; positive regulation of meiosis; male gonad development; positive regulation of peptidyl-serine phosphorylation; positive regulation of cGMP metabolic process; positive regulation of ossification; convergent extension involved in organogenesis; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; ameboidal cell migration; cartilage development; neural tube closure; epithelial to mesenchymal transition; hindgut morphogenesis; lung development; positive regulation of inflammatory response

Disease: Robinow Syndrome, Autosomal Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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