• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

WNT5B elisa kit :: Human wingless-type MMTV integration site family, member 5B ELISA Kit

Scan QR to view Datasheet
Catalog # MBS941658
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

wingless-type MMTV integration site family, member 5B (WNT5B), ELISA Kit

Popular Item
Also Known As

Human Protein Wnt-5b, WNT5B ELISA Kit

Product Synonym Names
Human Protein Wnt-5b (WNT5B) ELISA kit; MGC2648; WNT-5B protein; wingless-type MMTV integration site family; member 5B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS941658 Testing Data
Sequence Length
359
OMIM
606361
3D Structure
ModBase 3D Structure for Q9H1J7
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human WNT5B. No significant cross-reactivity or interference between human WNT5B and analogues was observed.
Samples
serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/ml -10 ng/ml
Sensitivity
<0.039 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WNT5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWNT5Bpurchase
MBS941658 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 5B (WNT5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT5B. The ELISA analytical biochemical technique of the MBS941658 kit is based on WNT5B antibody-WNT5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WNT5B elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of WNT5B elisa kit
WNT5B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS941658. Click to request current manual
NCBI/Uniprot data below describe general gene information for WNT5B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,323 Da
NCBI Official Full Name
protein Wnt-5b
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 5B
NCBI Official Symbol
WNT5B  [Similar Products]
NCBI Protein Information
protein Wnt-5b; WNT-5B protein
UniProt Protein Name
Protein Wnt-5b
UniProt Gene Name
WNT5B  [Similar Products]
UniProt Entry Name
WNT5B_HUMAN
NCBI Summary for WNT5B
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
UniProt Comments for WNT5B
WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: frizzled binding; receptor binding

Biological Process: fat cell differentiation; neuron differentiation; cell fate commitment; Wnt receptor signaling pathway; wound healing; chondrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withWNT5B elisa kit
 Reference Product  PubMed Publications
 PORCN elisa kit  >1 publications with WNT5B and PORCN
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000