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WNT9A elisa kit :: Human wingless-type MMTV integration site family, member 9A ELISA Kit

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Catalog # MBS942617
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

wingless-type MMTV integration site family, member 9A (WNT9A), ELISA Kit

Popular Item
Also Known As

Human Protein Wnt-9a, WNT9A ELISA Kit

Product Synonym Names
Human Protein Wnt-9a (WNT9A) ELISA kit; MGC138165; MGC141991; WNT14; wingless-type MMTV integration site family; member 14; wingless-type MMTV integration site family; member 9A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS942617 Testing Data
Sequence Length
365
OMIM
602863
3D Structure
ModBase 3D Structure for O14904
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human WNT9A. No significant cross-reactivity or interference between human WNT9A and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of human WNT9A is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of WNT9A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forWNT9Apurchase
MBS942617 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 9A (WNT9A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT9A. The ELISA analytical biochemical technique of the MBS942617 kit is based on WNT9A antibody-WNT9A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT9A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT9A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
WNT9A elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT9A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT9A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT9A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT9A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of WNT9A elisa kit
WNT9A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS942617. Click to request current manual
NCBI/Uniprot data below describe general gene information for WNT9A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,320 Da
NCBI Official Full Name
protein Wnt-9a
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 9A
NCBI Official Symbol
WNT9A  [Similar Products]
NCBI Official Synonym Symbols
WNT14
  [Similar Products]
NCBI Protein Information
protein Wnt-9a; wingless-type MMTV integration site family, member 14
UniProt Protein Name
Protein Wnt-9a
UniProt Synonym Protein Names
Protein Wnt-14
Protein Family
UniProt Gene Name
WNT9A  [Similar Products]
UniProt Synonym Gene Names
WNT14  [Similar Products]
UniProt Entry Name
WNT9A_HUMAN
NCBI Summary for WNT9A
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq, Jul 2008]
UniProt Comments for WNT9A
WNT9A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: frizzled binding

Biological Process: embryonic forelimb morphogenesis; Wnt receptor signaling pathway; cell fate commitment; multicellular organismal development; mitotic cell cycle checkpoint; negative regulation of chondrocyte differentiation; Wnt receptor signaling pathway through beta-catenin; negative regulation of caspase activity; embryonic skeletal morphogenesis; neuron differentiation; negative regulation of cell proliferation; cell-cell signaling; positive regulation of smoothened signaling pathway; positive regulation of cell differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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