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XIAP elisa kit :: Rat X-linked inhibitor of apoptosis ELISA Kit

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Catalog # MBS904012
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

X-linked inhibitor of apoptosis (XIAP), ELISA Kit

Also Known As

Rat Baculoviral IAP repeat-containing protein 4, XIAP ELISA Kit

Product Synonym Names
Rat Baculoviral IAP repeat-containing protein 4 (XIAP) ELISA kit; RP1-315G1.5; API3; BIRC4; ILP1; MIHA; XLP2; OTTHUMP00000196392; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; X-linked inhibitor of apoptosis
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS904012 Testing Data
Sequence Length
496
3D Structure
ModBase 3D Structure for Q9R0I6
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat XIAP. No significant cross-reactivity or interference between rat XIAP and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
The minimum detectable dose of rat XIAP is typically less than 5.88 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of XIAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forXIAPpurchase
MBS904012 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the X-linked inhibitor of apoptosis (XIAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing XIAP. The ELISA analytical biochemical technique of the MBS904012 kit is based on XIAP antibody-XIAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect XIAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, XIAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
XIAP elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for XIAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XIAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XIAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of XIAP elisa kit
XIAP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS904012. Click to request current manual
NCBI/Uniprot data below describe general gene information for XIAP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
56,073 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase XIAP
NCBI Official Synonym Full Names
X-linked inhibitor of apoptosis
NCBI Official Symbol
NCBI Official Synonym Symbols
Api3; Birc4; riap3
  [Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase XIAP; IAP-3; RIAP-3; X-linked IAP; IAP homolog A; apoptosis inhibitor protein 3; inhibitor of apoptosis protein 3; baculoviral IAP repeat-containing 4; X-linked inhibitor of apoptosis protein; baculoviral IAP repeat-containing protein 4
UniProt Protein Name
E3 ubiquitin-protein ligase XIAP
UniProt Synonym Protein Names
Baculoviral IAP repeat-containing protein 4; IAP homolog A; Inhibitor of apoptosis protein 3; IAP-3; rIAP-3; rIAP3; X-linked inhibitor of apoptosis protein; X-linked IAP
UniProt Gene Name
UniProt Synonym Gene Names
Api3; Birc4; IAP-3; rIAP-3; rIAP3; X-linked IAP  [Similar Products]
UniProt Entry Name
XIAP_RAT
NCBI Summary for XIAP
involved in directly inhibiting key apoptotic proteases, caspase 3 and 7 and is involved in maintenance of corpus luteum function and demise [RGD, Feb 2006]
UniProt Comments for XIAP
XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.

Protein type: Apoptosis; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Cellular Component: protein complex; perinuclear region of cytoplasm; spindle microtubule; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protease binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: neuron apoptosis; Wnt receptor signaling pathway; positive regulation of protein ubiquitination; protein ubiquitination; negative regulation of caspase activity; negative regulation of neuron apoptosis; response to DNA damage stimulus; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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