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YWHAH elisa kit :: Human 14 3 3 protein eta (YWHAH) ELISA Kit

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Catalog # MBS7245863
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Typical Testing Data/Standard Curve (for reference only)
Product Name

14 3 3 protein eta (YWHAH), ELISA Kit

Popular Item
Full Product Name

Human 14 3 3 protein eta (YWHAH) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS7245863 COA
OMIM
113508
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of YWHAH. No significant cross-reactivity or interference between YWHAH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAH and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of YWHAH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for YWHAHpurchase
MBS7245863 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 14 3 3 protein eta (YWHAH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YWHAH. The ELISA analytical biochemical technique of the MBS7245863 kit is based on YWHAH antibody-YWHAH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YWHAH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YWHAH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
YWHAH elisa kit
Intended Uses: This YWHAH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human YWHAH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: YWHAH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YWHAH antibody and an YWHAH-HRP conjugate. The assay sample and buffer are incubated together with YWHAH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAH concentration since YWHAH from samples and YWHAH-HRP conjugate compete for the anti-YWHAH antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAH from the sample, fewer sites are left to bind YWHAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAH concentration in each sample is interpolated from this standard curve.
Product Categories/Family for YWHAH elisa kit

Typical Testing Data/Standard Curve (for reference only) of YWHAH elisa kit
YWHAH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7245863. Click to request current manual
NCBI/Uniprot data below describe general gene information for YWHAH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
28,219 Da[Similar Products]
NCBI Official Full Name
14-3-3 protein eta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
NCBI Official Symbol
YWHAH  [Similar Products]
NCBI Official Synonym Symbols
YWHA1
  [Similar Products]
NCBI Protein Information
14-3-3 protein eta; 14-3-3 eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
UniProt Protein Name
14-3-3 protein eta
UniProt Synonym Protein Names
Protein AS1
Protein Family
UniProt Gene Name
YWHAH  [Similar Products]
UniProt Synonym Gene Names
YWHA1  [Similar Products]
UniProt Entry Name
1433F_HUMAN
NCBI Summary for YWHAH
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]
UniProt Comments for YWHAH
14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein domain specific binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding

Biological Process: intracellular protein transport; substantia nigra development; regulation of neuron differentiation; apoptosis; glucocorticoid receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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