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YWHAZ elisa kit :: Mouse tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide ELISA Kit

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Catalog # MBS917466
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ), ELISA Kit

Popular Item
Full Product Name

Mouse 14-3-3 protein zeta/delta, YWHAZ ELISA Kit

Product Synonym Names
Mouse 14-3-3 protein zeta/delta (YWHAZ) ELISA kit; 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; OTTHUMP00000165851; OTTHUMP00000165858; phospholipase A2; protein kinase C inhibitor protein-; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; zeta polypeptide
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS917466 Testing Data
3D Structure
ModBase 3D Structure for P63101
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse YWHAZ. No significant cross-reactivity or interference between Mouse YWHAZ and analogues was observed.
Assay Type
Sandwich
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of YWHAZ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for YWHAZpurchase
MBS917466 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YWHAZ. The ELISA analytical biochemical technique of the MBS917466 kit is based on YWHAZ antibody-YWHAZ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YWHAZ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YWHAZ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
YWHAZ elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAZ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of YWHAZ elisa kit
YWHAZ elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS917466. Click to request current manual
NCBI/Uniprot data below describe general gene information for YWHAZ. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,771 Da
NCBI Official Full Name
14-3-3 protein zeta/delta isoform 1
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
NCBI Official Symbol
Ywhaz  [Similar Products]
NCBI Official Synonym Symbols
AI596267; AL022924; AU020854; 14-3-3zeta; 1110013I11Rik
  [Similar Products]
NCBI Protein Information
14-3-3 protein zeta/delta; SEZ-2; KCIP-1; 14-3-3 zeta; protein kinase C inhibitor protein 1
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1; KCIP-1; SEZ-2
Protein Family
UniProt Gene Name
Ywhaz  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433Z_MOUSE
UniProt Comments for YWHAZ
14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: extracellular space; mast cell granule; focal adhesion; protein complex; mitochondrion; perinuclear region of cytoplasm; postsynaptic density; cytoplasm; leading edge; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein complex binding; transcription factor binding; protein kinase binding

Biological Process: response to drug; histamine secretion by mast cell; establishment of Golgi localization; protein targeting to mitochondrion; protein targeting
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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