Endomysial Antibody IgA (EMA IgA) ELISA Kit for Human

Principle of the Assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human EMA-IgA in samples. Add sample to wells pre-coated with one Human Endomysial IgA antigen, at same time add HRP-conjugated Human Endomysial IgA antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human EMA-IgA in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.

Small volumes of EMA IgA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS008634 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endomysial Antibody IgA (EMA IgA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EMA IgA. The ELISA analytical biochemical technique of the MBS008634 kit is based on EMA IgA antibody-EMA IgA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EMA IgA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EMA IgA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).