MyBioSource Life Science Resources

MyBioSource, Inc. is a biotechnological products distribution company formed to create a large portfolio of laboratory research reagents, both hard to find and common items, with worldwide distributions.

ELISA Testing

ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.

Western Blotting

Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. It uses hybridization techniques for the identification of the specific nucleic acids and genes.

Southern Blotting

The first of these techniques developed was the Southern blot, named after Dr. Edwin Southern who developed it to identify specific DNA sequences. Southern blotting is a detection technique used to find the target DNA sequences in the DNA sample in the field of molecular biology. The process starts from electrophoresis of DNA molecules which are hybridized in a blotting membrane followed by a transfer step where DNA from gel is transferred onto the blotting membrane.

Gene Knockout

When a mutation inactivates a gene function it is called as gene knockout. Gene knockout has its application both in classical genetics and modern techniques such as functional genomics. During the initial timescale, gene knockout was carried n transposon mutagenesis. The major disadvantage of that method is the laborious screening to find the knocked out gene.

Branched DNA Assay

The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by boosting the reporter signal, rather than replicating target sequences as the means of detection, and hence avoids the errors inherent in the extraction and amplification of target sequences.

Polymerase Chain Reaction

PCR is a technology which has its application in all molecular biology applications. The advent of PCR meant that insufficiencies in the quantity of DNA were no longer a limitation in molecular biology research or diagnostic procedures. The chemistry involved in PCR depends on the complementarity (matching) of the nucleotide bases in the double-stranded DNA helix. When a molecule of DNA is sufficiently heated, the hydrogen bonds holding together the double helix are disrupted and the molecule separates or denatures into single strands. If the DNA solution is allowed to cool, the complementary base pairs can reform to restore the original double helix.

Gene Transfer Technique

Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale experiment. However, the techniques of gene transfer in bacteria like Escherichia coli (E. coli) are simple and appropriate for the teaching and learning laboratory. Plasmid-based genetic transformation is the most common used technology for genetic transfer.

Recent Publications

National Poison Prevention Week

Accidental poisonings are a leading public health problem and cause of death from injuries in the United States as reported by the Centers for Disease Control and Prevention, National Center for Health Statistics (1). National Poison Prevention Week is designated in...

World TB Day

TB is primarily acquired following inhalation of aerosolized Mycobacterium tuberculosis bacilli and the majority of TB cases are pulmonary in nature. In 1882 Dr. Robert Koch announced his discovery of Mycobacterium tuberculosis, the bacillus that causes tuberculosis...

HIV/AIDS Awareness Day

HIV stands for Human Immunodeficiency Virus, which destroys the body's immune system, resulting in AIDS. HIV lives in blood or white blood cells. HIV infection began in the 1970s but scientists were unaware of its existence and today more than 35 million people across...

American Diabetes Alert Day

Diabetes affects about 9.4 percent of the U.S. population. A population of 7.2 million Americans which is almost 1 in 4 adults living with diabetes are unaware that they have the disease. Another 84 million Americans have prediabetes, where blood glucose levels are...

Epilepsy Awareness Day

Epilepsy which is a neurological condition affecting the nervous system, also known as a seizure disorder and can affect anyone with a brain. It is usually diagnosed after at least two seizures not caused by some known medical condition. Epileptic seizures are caused...

Brain Awareness Week

The human brain, a large mass of nerve tissue that’s protected within the skull is one of the most complex systems on the earth. Every component of the brain works together to keep the body functioning. Some of the main functions of the brain are processing sensory...

National Sleep Awareness Week

A good night’s sleep is an important aspect of your physical and mental health. Along with nutrition and exercise, sleep is also considered one of the important elements for healthy living.  Inadequate sleep can result in several medical problems such as diabetes and...

Patient Safety Awareness Week

The Health care system is organized by people where errors could happen. Although there are systems developed to reduce the errors they don’t necessarily prevent all mistakes. Some studies even suggest that medical error could have caused as many as 400,000 deaths in...

National Women and Girls HIV/AIDS Awareness Day

HIV and AIDS still continue to be a widespread public health issue with women particularly remaining vulnerable to the infection. HIV can be different in women when compared to men. For instance, a woman has a higher risk of getting the infection with vaginal sex,...

World Sleep Day

Sleep is an important part of a healthy living and inadequate sleep can result in various chronic medical problems such as heart disease, diabetes and even weight gain. Research also indicates an association between sleep restriction and negative changes in...

Trisomy awareness month

Trisomy is a chromosomal disorder when the affected individual has an additional chromosome, i.e, 47 chromosomes instead of 46. This abnormality is caused by the improper egg maturation and occurs spontaneously during conception. The random errors during cell division...

World Kidney Day

Globally an estimated 850 million people are suffering from kidney diseases and around 2.4 million deaths are the result of chronic kidney disease which is considered the 6th fastest growing cause of death. World Kidney Day is held on March 14th which helps raise...

Poison prevention – Attention for accidents

Accidental poisonings are a leading public health problem and cause of death from injuries in the United States as reported by the Centers for Disease Control and Prevention, National Center for Health Statistics (1). National Poison Prevention Week is designated in...

Multiple Sclerosis Awareness Month

In 1946, Sylvia Lawry started Multiple sclerosis (MS) movement with the founding of the National MS Society. By that time disease diagnosis took years to and there were no therapies to slow down the disease course. Also, research into multiple sclerosis was almost...

Kidney Health Month

Kidneys are the body’s chemical factories, filtering waste and performing vital functions that control red blood cell production and blood pressure. 1 in 9 American adults has kidney disease. Kidney disease risk can be addressed to a great extent by controlling blood...

National Nutrition Month

Adequate and proper nutrition is an important aspect of a healthy lifestyle for all individuals. The scope of public health practice is continuously expanding to promote sustainable health and nutrition. Across the globe, eating practices and the nutritive value of...

Endometriosis Awareness Month

Endometriosis is a debilitating gynecologic disease characterized by the presence of uterine epithelial and stromal tissues outside of the uterine cavity. with variable phenotypic and symptomatic presentation (1). Epidemiologic data show that in Canada, the inability...

National Colorectal Cancer Awareness Month

Colorectal cancer (CRC) is the second leading cause of cancer-related mortality worldwide and in the USA it represents the second reason for cancer-associated deaths. The incidence and mortality rates of CRC vary widely according to race and ethnicity (1). It is...

Bleeding Disorder Awareness Month

Bleeding disorders consist of a group of conditions where blood cannot clot properly. The body maintains blood in a fluid state in normal vessels by a complex process called Hemostasis. It generates a quick response at the site of vascular injury. This process...

Congenital Heart Defect Awareness Week

Congenital Heart Defect (CHD) affects the structure of the heart, the way it functions and the normal blood flow. There are around 18 different types of CHD recognized with several variations. CHD awareness week is observed every year from February 7-14 when awareness...

Eating Disorders Awareness and Screening Week

Eating Disorders describe illnesses include inadequate or excessive food intake which can ultimately damage an individual’s well-being. It is also characterized by severe distress or concern about body weight or shape. An eating disorder is also due to an unhealthy...

World Cancer Day

Cancer has a major impact on society and is one of the leading causes of death globally. It is considered a genetic disease developing with a mutation in the genes that can either occur because it is inherited from parents or can also rise during an individual’s...

International Prenatal Infection Prevention Month

Prenatal infection can occur because of a bacteria or virus that is passed onto the infant during pregnancy or at the time of delivery. Prenatal infection prevention is not only essential for the wellbeing of the infant but also equally important for the protection of...

National ‘Wear Red’ Day for Women’s Heart Health

Cardiovascular diseases continue to be one of the major health threat among women which in 80% of cases, it can be prevented. According to the Centers for Disease Control and Prevention (CDC), in the United States around 289,758 women in 2013 died of heart disease,...

American Heart Health Month

Heart disease is considered one of the common causes of death in the United States averaging at about 1 in 4 deaths. Heart diseases can affect individuals of any age and are now happening to younger adults also. And, according to Centers for Disease Control and...