MyBiosource Life Science Resources

MyBioSource, Inc. is a biotechnological products distribution company formed to create a large portfolio of laboratory research reagents, both hard to find and common items, with worldwide distributions.

ELISA Testing

ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.

Western Blotting

Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. It uses hybridization techniques for the identification of the specific nucleic acids and genes.

Southern Blotting

The first of these techniques developed was the Southern blot, named after Dr. Edwin Southern who developed it to identify specific DNA sequences. Southern blotting is a detection technique used to find the target DNA sequences in the DNA sample in the field of molecular biology. The process starts from electrophoresis of DNA molecules which are hybridized in a blotting membrane followed by a transfer step where DNA from gel is transferred onto the blotting membrane.

Gene Knockout

When a mutation inactivates a gene function it is called as gene knockout. Gene knockout has its application both in classical genetics and modern techniques such as functional genomics. During the initial timescale, gene knockout was carried n transposon mutagenesis. The major disadvantage of that method is the laborious screening to find the knocked out gene.

Branched DNA Assay

The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by boosting the reporter signal, rather than replicating target sequences as the means of detection, and hence avoids the errors inherent in the extraction and amplification of target sequences.

Polymerase Chain Reaction

PCR is a technology which has its application in all molecular biology applications. The advent of PCR meant that insufficiencies in the quantity of DNA were no longer a limitation in molecular biology research or diagnostic procedures. The chemistry involved in PCR depends on the complementarity (matching) of the nucleotide bases in the double-stranded DNA helix. When a molecule of DNA is sufficiently heated, the hydrogen bonds holding together the double helix are disrupted and the molecule separates or denatures into single strands. If the DNA solution is allowed to cool, the complementary base pairs can reform to restore the original double helix.

Gene Transfer Technique

Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale experiment. However, the techniques of gene transfer in bacteria like Escherichia coli (E. coli) are simple and appropriate for the teaching and learning laboratory. Plasmid-based genetic transformation is the most common used technology for genetic transfer.

Recent Publications

What is cockroach Immunotheraphy?

Cockroach Allergy Cockroaches are ubiquitous in nature and their infestations are a public health concern worldwide. These pests are common even in the cleanest of crowded urban areas and older dwellings. The proteins found in cockroach saliva are particularly...

Morgellons Disease

It is enough to make YOUR skin crawl Two groups, one from Bethesda, MD and the other from Modena, Italy, provide an interesting contrast in the scientific investigation of a rare and mysterious disease. They arrive at polar opposite conclusions about the origins and...

Good Fences Are Protective: New Understandings In Cancer Biology

For a few lucky gold miners, one tiny nugget of gold glinting on the ground reveals an entire gold mine. The same can be true in science. The most unlikely of findings can propel fields to entirely unexpected directions. A recent example is highlighted by work from a...

Protein Based Zika Vaccine Moves Into Clinical Trials

Protein Sciences reported that its protein based Zika vaccine candidate is expected to move on to human clinical trials by April. Also, the team developing the vaccine in Protein Sciences is now joined by the Institute of Technology in Immunobiological of the Oswaldo...

DNA Vaccines To Combat Zika Virus; NAID Push For Cure

An animated picture of zika virus vaccine which is commercially not available in the market right now.In November 2016, The World Health Organization (WHO) declared that Zika infections are here to stay like yellow fever and malaria, also spread by mosquitoes. WHO’s...