MyBiosource Life Science Resources

MyBioSource, Inc. is a biotechnological products distribution company formed to create a large portfolio of laboratory research reagents, both hard to find and common items, with worldwide distributions.

ELISA Testing

ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.

Western Blotting

Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. It uses hybridization techniques for the identification of the specific nucleic acids and genes.

Southern Blotting

The first of these techniques developed was the Southern blot, named after Dr. Edwin Southern who developed it to identify specific DNA sequences. Southern blotting is a detection technique used to find the target DNA sequences in the DNA sample in the field of molecular biology. The process starts from electrophoresis of DNA molecules which are hybridized in a blotting membrane followed by a transfer step where DNA from gel is transferred onto the blotting membrane.

Gene Knockout

When a mutation inactivates a gene function it is called as gene knockout. Gene knockout has its application both in classical genetics and modern techniques such as functional genomics. During the initial timescale, gene knockout was carried n transposon mutagenesis. The major disadvantage of that method is the laborious screening to find the knocked out gene.

Branched DNA Assay

The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by boosting the reporter signal, rather than replicating target sequences as the means of detection, and hence avoids the errors inherent in the extraction and amplification of target sequences.

Polymerase Chain Reaction

PCR is a technology which has its application in all molecular biology applications. The advent of PCR meant that insufficiencies in the quantity of DNA were no longer a limitation in molecular biology research or diagnostic procedures. The chemistry involved in PCR depends on the complementarity (matching) of the nucleotide bases in the double-stranded DNA helix. When a molecule of DNA is sufficiently heated, the hydrogen bonds holding together the double helix are disrupted and the molecule separates or denatures into single strands. If the DNA solution is allowed to cool, the complementary base pairs can reform to restore the original double helix.

Gene Transfer Technique

Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale experiment. However, the techniques of gene transfer in bacteria like Escherichia coli (E. coli) are simple and appropriate for the teaching and learning laboratory. Plasmid-based genetic transformation is the most common used technology for genetic transfer.

Recent Publications

World Cancer Day

Cancer has a major impact on society and is one of the leading causes of death globally. It is considered a genetic disease developing with a mutation in the genes that can either occur because it is inherited from parents or can also rise during an individual’s...

International Prenatal Infection Prevention Month

Prenatal infection can occur because of a bacteria or virus that is passed onto the infant during pregnancy or at the time of delivery. Prenatal infection prevention is not only essential for the wellbeing of the infant but also equally important for the protection of...

National ‘Wear Red’ Day for Women’s Heart Health

Cardiovascular diseases continue to be one of the major health threat among women which in 80% of cases, it can be prevented. According to the Centers for Disease Control and Prevention (CDC), in the United States around 289,758 women in 2013 died of heart disease,...

American Heart Health Month

Heart disease is considered one of the common causes of death in the United States averaging at about 1 in 4 deaths. Heart diseases can affect individuals of any age and are now happening to younger adults also. And, according to Centers for Disease Control and...

National Children’s Dental Health Month

Tooth decay is considered one of the most common chronic disease affecting children even though it is entirely preventable. Good oral hygiene habits can help children from getting cavities and in addition, dental sealants and fluoridated water are good ways to prevent...

National Winter Sports Traumatic Brain injury Awareness Month

Winter is a time when many people get involved in winter sports such as skiing, ice skating and snowmobiling which potential involves injuries just like any of the other sports. Although it is a fun activity, it could end up in a disaster if practiced without...

Cervical Health Awareness Month

Globally, majority of cervical cancer is found among women living in poverty without proper access to health care. In the U.S alone, around 13,000 women are diagnosed with cervical cancer annually and around 4,000 deaths occur as the result. Therefore, in an effort to...

National Birth Defects Prevention Month

According to the study by the Centers for Disease Control and Prevention (CDC) and the National Birth Defects Prevention Network, the birth defects occur in about 3% of all live births. It is estimated that every 4 ½ minutes, a child in the United States is born with...

Thyroid Awareness Week

January is observed as the thyroid awareness week to increase the awareness of thyroid associated diseases and to increase awareness among the elderly and pregnant women to have their thyroid test done periodically to avoid further complications. Thyroid is hormone...

Folic Acid Awareness Week

January 8-14, the first full week of January, marks Folic Acid Awareness Week. Folic Acid Awareness Week, sponsored by the National Birth Defects Prevention Network, highlights that the CDC and the U.S. Public Health Service recommend that all women between the ages...

National Glaucoma Awareness Month

It is estimated that around 3 million individuals in the United States have glaucoma and the National Eye Institute predicts that the number will reach 4.2 million by 2030 which is a 58% increase. And, according to the World Health Organization, around 4.5 million...

Angina

Angina, a relatively common condition, is a symptom of an underlying heart condition such as the coronary artery disease that occurs with reduced blood flow to the heart. This condition is often described as tightness or pain in the chest that also develops with the...

Chest Pain

Chest pain can develop because of a number of reasons some of which can be caused by benign conditions while others could be life-threatening requiring immediate medical intervention particularly when it involves the heart or the lungs. It is also considered one of...

Ovarian Cancer: New Studies Promises Better Survival Outcomes

 Ovarian cancer is considered the second most common gynecological malignancy, particularly in the western world. Generally, the ovarian cancer develops at the age of 40 but most develop after the age of 60. It is a difficult disease to treat and because of the...

Lung Cancer Claims More Lives than other Types of Cancer

Lung cancer is considered the leading cause of death among men and the second cause of death among women globally. It claims more lives than the ovarian, breast and colon cancers combined mainly because of the advanced stage of the disease at the time of diagnosis....

Brain tumor: Survival rates predominantly low

Brain cancer can either be primary or secondary meaning it has either originated in the brain or has started in another part of the body and spread to the brain. Among adults, secondary brain tumors are more common than the primary. Although there are various types of...

Thyroid Cancer can be cured in early Stages

Thyroid is a part of the endocrine system responsible for the production of various hormones that controls essential functions such as the heart, body temperature and the digestion. Thyroid cancer involves the thyroid gland which has two main types of cells; the...

Blood Cancer: A Growing Global Health Concern

Also known as hematologic cancers, it disrupts the normal production of blood cells and most of them develop in the bone marrow but can also affect the blood itself or the lymphatic system. Three main types of blood cancer are leukemia, lymphoma (Hodgkin lymphoma and...

Vaginal Cancer: Rare Gynecological Disease Affecting Women

Vaginal cancer commonly occurs among women between the ages of 50-70 affecting about 1 of every 1,100 women. Most vaginal cancer develops in the squamous cells which are the thin layer lining the vaginal wall. Cancer that has developed in the vagina is known as...

Stomach Cancer: One of the Deadliest Forms of Cancer

According to the World Health Organization, Stomach cancer is considered one of the third lethal cancers affecting people globally. Also known as Gastric cancer, it is divided into two classes; gastric cardia cancer and non-cardia gastric, i.e., cancer developing in...

Understanding the Current Trends on Different Types of Skin Cancer

Skin cancer is one of the common forms of cancer and most of them can be treated if detected in the early stage of the disease. In the US alone, annually more skin cancers are identified than any of the other cancers and have been growing over the decades. The common...

Pulmonary Hypertension – A less known problem

Pulmonary hypertension is defined as the mean pulmonary artery pressure ≥25 mm Hg at rest as determined by right heart catheterization 1. The upper level of normal for mean pulmonary artery pressure is 20 mm Hg. In simple terms it is elevated blood pressure in the...

GERD: A global health challenge

Gastroesophageal reflux disease (GERD) occurs when the reflux of stomach contents causes troublesome symptoms or complications [1]. A common medical problem which can be chronic as well with up to 40% of the population experiencing its symptoms at least once per...