Glycogen Phosphorylase Lsoenzyme Bb (GPBB) ELISA Kit for Human

Name: Human GPBB ELISA Kit; Glycogen Phosphorylase Lsoenzyme Bb; MyBioSource
Brand: MyBioSource
SKU: MBS009166
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS009166

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glycogen Phosphorylase Lsoenzyme Bb (GPBB), ELISA Kit

Full Product Name

Human Glycogen Phosphorylase Lsoenzyme Bb ELISA Kit

Product Gene Name

GPBB elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

0.625 ng/ml ~ 20 ng/ml.

Sensitivity

0.1 ng/ml.

Specificity

No significant cross-reactivity or interference between Human GPBB and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) are less than 15%.

Molecular Weight

96,696 Da

OMIM

138550

3D Structure

ModBase 3D Structure for P11216

Intended Uses

ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate GPBB concentrations in Human serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications

Other Notes

Small volumes of GPBB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPBB purchase

MBS009166 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycogen Phosphorylase Lsoenzyme Bb (GPBB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPBB. The ELISA analytical biochemical technique of the MBS009166 kit is based on GPBB antibody-GPBB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPBB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPBB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPBB. It may not necessarily be applicable to this product.

NCBI GI #

21361370

NCBI GeneID

5834

NCBI Accession #

NP_002853.2

UniProt Primary Accession #

P11216

UniProt Secondary Accession #

Q96AK1; Q9NPX8

UniProt Related Accession #

P11216

GenBank Nucleotide #

NM_002862.3

NCBI Official Full Name

glycogen phosphorylase, brain form

NCBI Official Synonym Full Names

phosphorylase, glycogen; brain

NCBI Official Symbol

PYGB

NCBI Official Synonym Symbols

GPBB

UniProt Protein Name

Glycogen phosphorylase, brain form

UniProt Gene Name

PYGB

UniProt Entry Name

PYGB_HUMAN

NCBI Protein Information

glycogen phosphorylase, brain form; glycogen phosphorylase B

NCBI Summary for GPBB

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

UniProt Comments for GPBB

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: Phosphorylase; Kinase, other; Transferase; Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Research Articles on GPBB

1. GPBB is a valuable biological marker to predict the prognosis in patient with acute coronary syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPBB elisa kit

Reference Product	PubMed Publications
PGM1 elisa kit	>1 publications with GPBB and PGM1
PHKB elisa kit	>1 publications with GPBB and PHKB

Pathways associated with GPBB elisa kit

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycogen Metabolism Pathway antibodies	Glycogen Metabolism Pathway Diagram
Glycogen Breakdown (glycogenolysis) Pathway antibodies	Glycogen Breakdown (glycogenolysis) Pathway Diagram
Glycogen Storage Diseases Pathway antibodies	Glycogen Storage Diseases Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
Myoclonic Epilepsy Of Lafora Pathway antibodies	Myoclonic Epilepsy Of Lafora Pathway Diagram

Diseases associated with GPBB elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>3 publications with GPBB and Neoplasms
Inflammation Antibodies	>2 publications with GPBB and Inflammation
Brain Diseases Antibodies	>1 publications with GPBB and Brain Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with GPBB and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with GPBB and Liver Diseases
Disease Models, Animal Antibodies	>1 publications with GPBB and Disease Models, Animal
Breast Neoplasms Antibodies	>1 publications with GPBB and Breast Neoplasms
Nervous System Diseases Antibodies	>1 publications with GPBB and Nervous System Diseases
Liver Neoplasms Antibodies	>1 publications with GPBB and Liver Neoplasms
Kidney Diseases Antibodies	>1 publications with GPBB and Kidney Diseases

Organs/Tissues associated with GPBB elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with GPBB and Brain
Liver Antibodies	>2 publications with GPBB and Liver
Blood Antibodies	>2 publications with GPBB and Blood
Muscle Antibodies	>2 publications with GPBB and Muscle
Vascular Antibodies	>1 publications with GPBB and Vascular
Heart Antibodies	>1 publications with GPBB and Heart
Kidney Antibodies	>1 publications with GPBB and Kidney
Pancreas Antibodies	>1 publications with GPBB and Pancreas
Skin Antibodies	>1 publications with GPBB and Skin