Ferritin (FE) ELISA Kit for Chicken

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated FE concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken FE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FE and FE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of FE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS035198 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ferritin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FE. The ELISA analytical biochemical technique of the MBS035198 kit is based on FE antibody-FE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Sandwich ELISA assays have been a cell research and molecular biology staple for 25 years, But only with the advent of more specific primary antibodies has detection of species specific ubiquitous expressed proteins like ferritin been achieved. MyBiosource's chicken ferritin FE ELISA kit recognizes ng ml concentrations in serum plasma or cell culture sample lysates with no cross reactivity to human ferritin (see human ELISA kit: product categories proteins ELISAs). Our ELISA kits are shipped complete with all products and reagents required for your samples such as 1o antibody plate, secondary antibodies with AMP or HRP conjugate, standard curve protein product and ALEXA 174 fluor detection. Easy of performance allows for ferritin ELISA assay arrays series to be established. For assistance in custom design, use our ELISA kits reagents customer service. Check out our economical and top quality lines of alphabetical list antibodies reagents peptides protein labeling kits services.