Collagen Auto (CLA) ELISA Kit for Human

Name: Human CLA ELISA Kit; Collagen Auto; MyBioSource
Brand: MyBioSource
SKU: MBS038839
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS038839

Principle of the Assay: The kit uses a indirect enzyme-linked immunosorbent assay (ELISA) to qualitatively analyze Human Collagen Autoantibody (Anti-CL) in Human serum or plasma samples. This kit is in vitro research use only! Not for therapeutic or test… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Collagen Autoantibody (CLA), ELISA Kit

Full Product Name

Human Collagen Autoantibody ELISA Kit

Product Gene Name

CLA elisa kit

Species Reactivity

Human

Samples

Human serum or plasma samples

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (?) are less than 15%.

Intra-assay Precision

Intra-assay CV (%) are less than 15%.

Molecular Weight

64,771 Da

OMIM

610004

3D Structure

ModBase 3D Structure for Q9BXS0

Other Notes

Small volumes of CLA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLA purchase

MBS038839 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Autoantibody (CLA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLA. The ELISA analytical biochemical technique of the MBS038839 kit is based on CLA antibody-CLA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CLA. It may not necessarily be applicable to this product.

NCBI GI #

38570075

NCBI GeneID

84570

NCBI Accession #

NP_942014.1

UniProt Primary Accession #

Q9BXS0

UniProt Secondary Accession #

Q9BXR9; A8MPZ6

UniProt Related Accession #

Q9BXS0

GenBank Nucleotide #

NM_198721.3

NCBI Official Full Name

collagen alpha-1(XXV) chain isoform 1

NCBI Official Synonym Full Names

collagen, type XXV, alpha 1

NCBI Official Symbol

COL25A1

NCBI Official Synonym Symbols

AMY; CLAC; CLACP; CLAC-P

UniProt Protein Name

Collagen alpha-1(XXV) chain

UniProt Synonym Protein Names

Alzheimer disease amyloid-associated protein; AMY; CLAC-P

Protein Family

Ribonuclease clavin

UniProt Gene Name

COL25A1

UniProt Synonym Gene Names

AMY; CLAC

UniProt Entry Name

COPA1_HUMAN

NCBI Protein Information

collagen alpha-1(XXV) chain; collagen alpha-1(XXV) chain; alzheimer disease amyloid-associated protein; collagenous Alzheimer amyloid plaque component; collagen-like Alzheimer amyloid plaque component

NCBI Summary for CLA

This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for CLA

COL25A1: Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; collagen; endoplasmic reticulum lumen; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function: heparin binding; beta-amyloid binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis

Disease: Fibrosis Of Extraocular Muscles, Congenital, 5

Research Articles on CLA

1. COL25A1 methylation correlates with severity of cervical intraepithelial neoplasia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CLA elisa kit

Products by Pathway	Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with CLA elisa kit

Disease Name	Pubmed Publications
Neurobehavioral Manifestations Antibodies	>1 publications with CLA and Neurobehavioral Manifestations
Congenital Abnormalities Antibodies	>1 publications with CLA and Congenital Abnormalities
Abnormalities, Multiple Antibodies	>1 publications with CLA and Abnormalities, Multiple
Uterine Cervical Neoplasms Antibodies	>1 publications with CLA and Uterine Cervical Neoplasms

Organs/Tissues associated with CLA elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>6 publications with CLA and Brain
Muscle Antibodies	>1 publications with CLA and Muscle
Kidney Antibodies	>1 publications with CLA and Kidney