Dihydropyrimidine Dehydrogenase (DPYD) ELISA Kit for Human

Name: Human DPYD ELISA Kit; Dihydropyrimidine Dehydrogenase; MyBioSource
Brand: MyBioSource
SKU: MBS041319
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS041319

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of DPYD (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Dihydropyrimidine Dehydrogenase (DPYD), ELISA Kit

Full Product Name

Human Dihydropyrimidine Dehydrogenase ELISA Kit

Product Gene Name

DPYD elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Detection Range

3.12ng/ml-100ng/ml

Sensitivity

1.0ng/ml

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

111,401 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (97543299..98386615, complement). Location: 1p22

OMIM

274270

3D Structure

ModBase 3D Structure for Q12882

Other Notes

Small volumes of DPYD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPYD purchase

MBS041319 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dihydropyrimidine Dehydrogenase (DPYD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPYD. The ELISA analytical biochemical technique of the MBS041319 kit is based on DPYD antibody-DPYD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPYD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPYD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DPYD. It may not necessarily be applicable to this product.

NCBI GI #

237757300

NCBI GeneID

1806

NCBI Accession #

NP_001153773.1

UniProt Primary Accession #

Q12882

UniProt Secondary Accession #

Q16694; Q16761; Q32NB0; Q96HL6; Q96TH1; A2RRQ2; A2RRQ3; A8K5A2; A8MWG9; B1AN21; E9PFN1

UniProt Related Accession #

Q12882

GenBank Nucleotide #

NM_001160301.1

NCBI Official Full Name

dihydropyrimidine dehydrogenase

NCBI Official Synonym Full Names

dihydropyrimidine dehydrogenase

NCBI Official Symbol

DPYD

NCBI Official Synonym Symbols

DHP; DPD; DHPDHASE

UniProt Protein Name

Dihydropyrimidine dehydrogenase [NADP(+)]

UniProt Synonym Protein Names

Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

Protein Family

Dihydropyrimidine dehydrogenase

UniProt Gene Name

DPYD

UniProt Synonym Gene Names

DHPDHase; DPD

UniProt Entry Name

DPYD_HUMAN

NCBI Protein Information

dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase

NCBI Summary for DPYD

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

UniProt Comments for DPYD

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Other Amino Acids Metabolism - beta-alanine; EC 1.3.1.2; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; FAD binding; dihydroorotate dehydrogenase activity; 4 iron, 4 sulfur cluster binding; metal ion binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; UMP biosynthetic process; uracil catabolic process; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; thymidine catabolic process; pyrimidine base catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency

Research Articles on DPYD

1. Data suggest that upregulation of dihydropyrimidine dehydrogenase (DPD) in liver may be involved in acquired resistance to 5-fluorouracil (5-FU), and DPD inhibitors or increasing 5-FU dosage may overcome 5-FU acquired resistance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DPYD elisa kit

Reference Product	PubMed Publications
TYMP elisa kit	>3 publications with DPYD and TYMP
CYP2A6 elisa kit	>2 publications with DPYD and CYP2A6
UPB1 elisa kit	>2 publications with DPYD and UPB1
UPP1 elisa kit	>1 publications with DPYD and UPP1
UMPS elisa kit	>1 publications with DPYD and UMPS
DPYS elisa kit	>1 publications with DPYD and DPYS

Pathways associated with DPYD elisa kit

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Pantothenate And CoA Biosynthesis Pathway antibodies	Pantothenate And CoA Biosynthesis Pathway Diagram
Pantothenate And CoA Biosynthesis Pathway antibodies	Pantothenate And CoA Biosynthesis Pathway Diagram
Pyrimidine Catabolism Pathway antibodies	Pyrimidine Catabolism Pathway Diagram
Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway antibodies	Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway Diagram
Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway antibodies	Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway Diagram

Diseases associated with DPYD elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>100 publications with DPYD and Neoplasms
Drug Toxicity Antibodies	>79 publications with DPYD and Drug Toxicity
Dihydropyrimidine Dehydrogenase Deficiency Antibodies	>66 publications with DPYD and Dihydropyrimidine Dehydrogenase Deficiency
Gastrointestinal Neoplasms Antibodies	>52 publications with DPYD and Gastrointestinal Neoplasms
Gastrointestinal Diseases Antibodies	>52 publications with DPYD and Gastrointestinal Diseases
Colorectal Neoplasms Antibodies	>43 publications with DPYD and Colorectal Neoplasms
Carcinoma Antibodies	>30 publications with DPYD and Carcinoma
Adenocarcinoma Antibodies	>15 publications with DPYD and Adenocarcinoma
Breast Neoplasms Antibodies	>14 publications with DPYD and Breast Neoplasms
Purine-Pyrimidine Metabolism, Inborn Errors Antibodies	>14 publications with DPYD and Purine-Pyrimidine Metabolism, Inborn Errors

Organs/Tissues associated with DPYD elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>61 publications with DPYD and Blood
Bone Antibodies	>29 publications with DPYD and Bone
Liver Antibodies	>11 publications with DPYD and Liver
Connective Tissue Antibodies	>10 publications with DPYD and Connective Tissue
Lung Antibodies	>7 publications with DPYD and Lung
Prostate Antibodies	>6 publications with DPYD and Prostate
Skin Antibodies	>5 publications with DPYD and Skin
Kidney Antibodies	>4 publications with DPYD and Kidney
Brain Antibodies	>4 publications with DPYD and Brain
Nerve Antibodies	>4 publications with DPYD and Nerve