Cytochrome P450 7a1 (CYP7A1) ELISA Kit for Human

Name: Human CYP7A1 ELISA Kit; Cytochrome P450 7a1; MyBioSource
Brand: MyBioSource
SKU: MBS042560
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS042560

MBS042560 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 7A1 (CYP7A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Cytochrome P450 7A1 (CYP7A1), ELISA Kit

Full Product Name

Human Cytochrome P450 7A1 ELISA Kit

Product Gene Name

CYP7A1 elisa kit

Species Reactivity

Human

Samples

Undiluted original human serum, plasma or tissue homogenates samples

Detection Range

0.625ng/ml-20ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

57,661 Da

OMIM

118455

3D Structure

ModBase 3D Structure for P22680

Other Notes

Small volumes of CYP7A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP7A1. It may not necessarily be applicable to this product.

NCBI GI #

166295200

NCBI GeneID

1581

NCBI Accession #

NP_000771.2

UniProt Primary Accession #

P22680

UniProt Secondary Accession #

P78454; Q3MIL8; Q7KZ19

UniProt Related Accession #

P22680

GenBank Nucleotide #

NM_000780.3

NCBI Official Full Name

cholesterol 7-alpha-monooxygenase

NCBI Official Synonym Full Names

cytochrome P450, family 7, subfamily A, polypeptide 1

NCBI Official Symbol

CYP7A1

NCBI Official Synonym Symbols

CP7A; CYP7; CYPVII

UniProt Protein Name

Cholesterol 7-alpha-monooxygenase

UniProt Synonym Protein Names

CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1

Protein Family

Cholesterol 7-alpha-monooxygenase

UniProt Gene Name

CYP7A1

UniProt Synonym Gene Names

CYP7

UniProt Entry Name

CP7A1_HUMAN

NCBI Protein Information

cholesterol 7-alpha-monooxygenase; cytochrome P450 7A1; cholesterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIA polypeptide 1

NCBI Summary for CYP7A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]

UniProt Comments for CYP7A1

CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: Endoplasmic reticulum; EC 1.14.13.17; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 8q11-q12

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: cholesterol 7-alpha-monooxygenase activity; iron ion binding; heme binding

Biological Process: bile acid biosynthetic process; cholesterol homeostasis; bile acid metabolic process; xenobiotic metabolic process; cellular lipid metabolic process; cholesterol catabolic process; sterol metabolic process; transmembrane transport

Research Articles on CYP7A1

1. Activation of the VDR represses hepatic SHP to increase levels of CYP7A1 and reduce cholesterol.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYP7A1 elisa kit

Reference Product	PubMed Publications
CYP27A1 elisa kit	>83 publications with CYP7A1 and CYP27A1
NR5A2 elisa kit	>20 publications with CYP7A1 and NR5A2
NR0B2 elisa kit	>8 publications with CYP7A1 and NR0B2
PPARA elisa kit	>4 publications with CYP7A1 and PPARA
NR1H4 elisa kit	>4 publications with CYP7A1 and NR1H4
POR elisa kit	>2 publications with CYP7A1 and POR
HSD3B2 elisa kit	>2 publications with CYP7A1 and HSD3B2
HSD3B7 elisa kit	>2 publications with CYP7A1 and HSD3B7

Pathways associated with CYP7A1 elisa kit

Products by Pathway	Pathway Diagram
Bile Acid And Bile Salt Metabolism Pathway antibodies	Bile Acid And Bile Salt Metabolism Pathway Diagram
Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies	Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies	Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Cytochrome P450 - Arranged By Substrate Type Pathway antibodies	Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
Drug Induction Of Bile Acid Pathway antibodies	Drug Induction Of Bile Acid Pathway Diagram
Endogenous Sterols Pathway antibodies	Endogenous Sterols Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram

Diseases associated with CYP7A1 elisa kit

Disease Name	Pubmed Publications
Hypercholesterolemia Antibodies	>198 publications with CYP7A1 and Hypercholesterolemia
Fatty Liver Antibodies	>192 publications with CYP7A1 and Fatty Liver
Dyslipidemias Antibodies	>189 publications with CYP7A1 and Dyslipidemias
Hyperlipidemias Antibodies	>180 publications with CYP7A1 and Hyperlipidemias
Liver Diseases Antibodies	>179 publications with CYP7A1 and Liver Diseases
Atherosclerosis Antibodies	>135 publications with CYP7A1 and Atherosclerosis
Cardiovascular Diseases Antibodies	>93 publications with CYP7A1 and Cardiovascular Diseases
Cholestasis Antibodies	>92 publications with CYP7A1 and Cholestasis
Neoplasms Antibodies	>62 publications with CYP7A1 and Neoplasms
Liver Neoplasms Antibodies	>54 publications with CYP7A1 and Liver Neoplasms

Organs/Tissues associated with CYP7A1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1417 publications with CYP7A1 and Liver
Testis Antibodies	>7 publications with CYP7A1 and Testis