Escherichia Coli arfA Recombinant Protein – Cat# MBS1011348

Alternative Ribosome-Rescue Factor A (arfA) Escherichia Coli Recombinant Protein

Name: arfA Recombinant Protein; Escherichia Coli; MyBioSource
Brand: MyBioSource
SKU: MBS1011348
Price: 680 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1011348

MBS1011348 is a recombinant protein targeting arfA. Also known as Recombinant Escherichia coli Alternative ribosome-rescue factor A (arfA). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$680 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alternative ribosome-rescue factor A (arfA), Recombinant Protein

Full Product Name

Recombinant Escherichia coli Alternative ribosome-rescue factor A (arfA)

Product Gene Name

arfA recombinant protein

Sequence

MSRYQHTKGQ IKDNAIEALL HDPLFRQRVE KNKKGKGSYM RKGKHGNRGN WEASGKKVNH FFTTGLLLSG AC

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

8,171 Da

Sequence Positions

1-72, full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for P36675

Species

Escherichia coli (strain K12)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of arfA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for arfA. It may not necessarily be applicable to this product.

NCBI GI #

446014841

NCBI Accession #

WP_000092696.1

UniProt Primary Accession #

P36675

UniProt Secondary Accession #

Q2EET4; Q2M6V6

GenBank Nucleotide #

WP_000092696.1

NCBI Official Full Name

MULTISPECIES: ribosome alternative rescue factor ArfA

UniProt Protein Name

Alternative ribosome-rescue factor A

Protein Family

Alternative ribosome-rescue factor

UniProt Gene Name

arfA

UniProt Comments for arfA

Rescues ribosomes stalled at the 3' end of non-stop mRNAs (PubMed:21062370, PubMed:21435036). This activity is crucial when the stalled ribosome cannot be rescued by the SsrA(tmRNA)-SmpB quality control system (PubMed:21062370, PubMed:21435036). Binds the 30S subunit, contacting 16S rRNA with the N-terminus near the decoding center and its C-terminus in the mRNA entry channel; contacts change in the presence of release factor 2 (RF2, also named PrfB) (PubMed:25355516, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Requires RF2/PrfB to hydrolyze stalled peptidyl-tRNA on the ribosome; recruits and probably helps position RF2/PrfB correctly in the ribosomal A site so RF2's GGQ motif can hydrolyze the peptidyl-tRNA bond (PubMed:22857598, PubMed:22922063, PubMed:25355516, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Does not release ribosomes with a programmed pause caused by regulatory chains such as SecM-mediated pausing (PubMed:22857598). Binds tRNA which may stimulate its ribosome rescue activity (PubMed:22857598).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with arfA recombinant protein

Reference Product	PubMed Publications
rmf recombinant protein	>1 publications with arfA and rmf