Escherichia Coli dpiB Recombinant Protein – Cat# MBS1013462

Sensor Histidine Kinase Dpib (dpiB) Escherichia Coli Recombinant Protein

Name: dpiB Recombinant Protein; Escherichia Coli; MyBioSource
Brand: MyBioSource
SKU: MBS1013462
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1013462

MBS1013462 is a recombinant protein targeting dpiB. Also known as Recombinant Escherichia coli Sensor histidine kinase DpiB (dpiB). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Sensor histidine kinase DpiB (dpiB), Recombinant Protein

Full Product Name

Recombinant Escherichia coli Sensor histidine kinase DpiB (dpiB)

Product Gene Name

dpiB recombinant protein

Product Synonym Gene Name

dpiB; citA, mpdB, ybeP

Product Synonym Names

Recombinant Sensor histidine kinase DpiB (dpiB); Sensor histidine kinase DpiB EC= 2.7.13.3; Sensor histidine kinase CitA

Sequence

MLQLNENKQF AFFQRLAFPL RIFLLILVFS IFVIAALAQY FTASFEDYLT LHVRDMAMNQ AKIIASNDSV ISAVKTRDYK RLATIANKLQ RDTDFDYVVI GDRHSIRLYH PNPEKIGYPM QFTKQGALEK GESYFITGKG SMGMAMRAKT PIFDDDGKVI GVVSIGYLVS KIDSWRAEFL LPMAGVFVVL LGILMLLSWF LAAHIRRQMM GMEPKQIARV VRQQEALFSS VYEGLIAVDP HGYITAINRN ARKMLGLSSP GRQWLGKPIV EVVRPADFFT EQIDEKRQDV VANFNGLSVI ANREAIRSGD DLLGAIISFR SKDEISTLNA QLTQIKQYVE SLRTLRHEHL NWMSTLNGLL QMKEYDRVLA MVQGESQAQQ QLIDSLREAF ADRQVAGLLF GKVQRARELG LKMIIVPGSQ LSQLPPGLDS TEFAAIVGNL LDNAFEASLR SDEGNKIVEL FLSDEGDDVV IEVADQGCGV PESLRDKIFE QGVSTRADEP GEHGIGLYLI ASYVTRCGGV ITLEDNDPCG TLFSIYIPKV KPNDSSINPI DR

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

61,684 Da

Sequence Positions

1-552

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

NC_000913.2 (651458..653116)

3D Structure

ModBase 3D Structure for P77510

Species

Escherichia coli (strain K12)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of dpiB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for dpiB. It may not necessarily be applicable to this product.

NCBI GI #

16128602

NCBI GeneID

945233

NCBI Accession #

NP_415152.1

UniProt Primary Accession #

P77510

UniProt Secondary Accession #

O54338

GenBank Nucleotide #

NC_000913.2

NCBI Official Full Name

sensory histidine kinase in two-component regulatory system with citB

NCBI Official Symbol

dpiB

NCBI Official Synonym Symbols

citA; ECK0612; JW0611; mpdB; ybeP

UniProt Protein Name

Sensor histidine kinase DpiB

UniProt Synonym Protein Names

Sensor histidine kinase CitA

Protein Family

Sensor histidine kinase

UniProt Gene Name

dpiB

UniProt Synonym Gene Names

citA; mpdB; ybeP

UniProt Entry Name

DPIB_ECOLI

NCBI Protein Information

sensory histidine kinase in two-component regulatory system with citB

NCBI Summary for dpiB

An amber mutation has been generated . [More information is available at EcoCyc: G6345].

UniProt Comments for dpiB

Function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Functions as a sensor kinase that phosphorylates DpiA in the presence of citrate. Ref.6 Ref.8

Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulation: Autophosphorylation is induced in vitro by dithiothreitol (DTT). Ref.8

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.7.

Domain: The periplasmic domain binds citrate with high affinity. The C-terminal cytoplasmic domain senses reduced conditions using a single Cys in vitro. Ref.6 Ref.8

Post-translational modification: Autophosphorylated. Ref.8

Sequence similarities: Contains 1 histidine kinase domain.Contains 1 PAS (PER-ARNT-SIM) domain.

Research Articles on dpiB

1. DcuS/DcuR modulating citA and citB expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with dpiB recombinant protein

Reference Product	PubMed Publications
dpiA recombinant protein	>8 publications with dpiB and dpiA
citC recombinant protein	>3 publications with dpiB and citC
citE recombinant protein	>1 publications with dpiB and citE
citD recombinant protein	>1 publications with dpiB and citD
citT recombinant protein	>1 publications with dpiB and citT
dcuR recombinant protein	>1 publications with dpiB and dcuR

Pathways associated with dpiB recombinant protein

Products by Pathway	Pathway Diagram
CitA-CitB (citrate Fermentation) Two-component Regulatory System Pathway antibodies	CitA-CitB (citrate Fermentation) Two-component Regulatory System Pathway Diagram
CitA-CitB (citrate Fermentation) Two-component Regulatory System Pathway antibodies	CitA-CitB (citrate Fermentation) Two-component Regulatory System Pathway Diagram
DpiBA Two-Component Signal Transduction System Pathway antibodies	DpiBA Two-Component Signal Transduction System Pathway Diagram
Two-component System Pathway antibodies	Two-component System Pathway Diagram
Two-component System Pathway antibodies	Two-component System Pathway Diagram