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4-Hydroxyphenylacetaldehyde Oxime Monooxygenase (CYP71E1) Sorghum Bicolor Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1021647

MBS1021647 is a recombinant protein targeting CYP71E1. Also known as Recombinant Sorghum bicolor 4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. CYP71E1 More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1), Recombinant Protein
Full Product Name
Recombinant Sorghum bicolor 4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1)
Product Synonym Gene Name
Recombinant 4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1); 4-hydroxyphenylacetaldehyde oxime monooxygenase EC= 1.14.13.68; Cytochrome P450 71E1
Sequence
MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG GAPRLPPGPA QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV VVSSAEAARE VLKVHDVDCC SRPASPGPKR LSYDLKNVGF APYGEYWREM RKLFALELLS MRRVKAACYA REQEMDRLVA DLDRAAASKA SIVLNDHVFA LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF SAEDFFPNAA GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV LRKAQAEVRA AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR ETMRDTTICG YDVPANTRVF VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD YYGSHFELIP FGAGRRICPG LTMGETNVTF TLANLLYCYD WALPGAMKPE DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
59,088 Da
Sequence Positions
1-531
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for O48958
Species
Sorghum bicolor (Sorghum) (Sorghum vulgare)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CYP71E1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP71E1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
4-hydroxyphenylacetaldehyde oxime monooxygenase
UniProt Protein Name
4-hydroxyphenylacetaldehyde oxime monooxygenase
UniProt Synonym Protein Names
Cytochrome P450 71E1
UniProt Gene Name
CYP71E1
UniProt Entry Name
C71E1_SORBI

UniProt Comments for CYP71E1

Function: Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.

Catalytic activity: (Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O.

Cofactor: Heme group

By similarity.

Pathway: Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 2/3.

Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein

Potential.

Sequence similarities: Belongs to the cytochrome P450 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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