G Protein Coupled Receptor 120 (GPR120) ELISA Kit for Rat

Name: Rat GPR120 ELISA Kit; G Protein Coupled Receptor 120; MyBioSource
Brand: MyBioSource
SKU: MBS102225
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS102225

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GPR120 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPR120 antibody to coat Microelisa Stripplate wells… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

G Protein Coupled Receptor 120 (GPR120), ELISA Kit

Full Product Name

Rat G Protein Coupled Receptor 120 ELISA Kit

Product Gene Name

GPR120 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

3.12 ng/ml - 100 ng/ml.

Sensitivity

1.0 ng/ml.

Specificity

No significant cross-reactivity or interference between Rat GPR120 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) are less than 15%.

Molecular Weight

42,241 Da

OMIM

607514

3D Structure

ModBase 3D Structure for Q5NUL3

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GPR120 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPR120 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPR120 and GPR120 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPR120 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of GPR120 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPR120 purchase

MBS102225 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G Protein Coupled Receptor 120 (GPR120) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPR120. The ELISA analytical biochemical technique of the MBS102225 kit is based on GPR120 antibody-GPR120 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPR120 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPR120. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPR120. It may not necessarily be applicable to this product.

NCBI GI #

72533526

NCBI GeneID

338557

NCBI Accession #

AAI01176.1

UniProt Primary Accession #

Q5NUL3

UniProt Secondary Accession #

Q495H1; Q5VY25; Q5VY26; Q7Z605; Q86SM7

UniProt Related Accession #

Q5NUL3

NCBI Official Full Name

GPR120 protein

NCBI Official Synonym Full Names

free fatty acid receptor 4

NCBI Official Symbol

FFAR4

NCBI Official Synonym Symbols

GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1

UniProt Protein Name

Free fatty acid receptor 4

UniProt Synonym Protein Names

G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; Omega-3 fatty acid receptor 1

Protein Family

Free fatty acid receptor

UniProt Gene Name

FFAR4

UniProt Synonym Gene Names

GPR120; GPR129; O3FAR1; PGR4

UniProt Entry Name

FFAR4_HUMAN

NCBI Protein Information

free fatty acid receptor 4; omega-3 fatty acid receptor 1; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4

NCBI Summary for GPR120

This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

UniProt Comments for GPR120

GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: endocytic vesicle; integral to plasma membrane; plasma membrane

Molecular Function: peptide binding; fatty acid binding; taste receptor activity

Biological Process: hormone secretion; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; negative regulation of cytokine secretion; detection of chemical stimulus involved in sensory perception of taste; cellular response to hormone stimulus; negative regulation of apoptosis

Disease: Body Mass Index Quantitative Trait Locus 10

Research Articles on GPR120

1. this study demonstrates the expression of GPR120 in pancreas and shows the distribution of GPR120 in human and rat pancreas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPR120 elisa kit

Reference Product	PubMed Publications
FFAR1 elisa kit	>2 publications with GPR120 and FFAR1
FFAR2 elisa kit	>1 publications with GPR120 and FFAR2
GPR119 elisa kit	>1 publications with GPR120 and GPR119
GCG elisa kit	>1 publications with GPR120 and GCG

Pathways associated with GPR120 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Free Fatty Acid Receptors Pathway antibodies	Free Fatty Acid Receptors Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Incretin Synthesis, Secretion, And Inactivation Pathway antibodies	Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Peptide Hormone Metabolism Pathway antibodies	Peptide Hormone Metabolism Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram
Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway antibodies	Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway Diagram

Diseases associated with GPR120 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>6 publications with GPR120 and Inflammation
Fatty Liver Antibodies	>2 publications with GPR120 and Fatty Liver
Atherosclerosis Antibodies	>1 publications with GPR120 and Atherosclerosis

Organs/Tissues associated with GPR120 elisa kit

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>1 publications with GPR120 and Intestine