Vaccinia Virus VPK1 Recombinant Protein – Cat# MBS1023993

Serine/Threonine-Protein Kinase 1 (VPK1) Vaccinia Virus Recombinant Protein

Name: VPK1 Recombinant Protein; Vaccinia Virus; MyBioSource
Brand: MyBioSource
SKU: MBS1023993
Price: 990 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1023993

MBS1023993 is a recombinant protein targeting VPK1. Also known as Recombinant Vaccinia virus Serine/threonine-protein kinase 1 (VPK1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$990 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Serine/threonine-protein kinase 1 (VPK1), Recombinant Protein

Full Product Name

Recombinant Vaccinia virus Serine/threonine-protein kinase 1 (VPK1)

Product Gene Name

VPK1 recombinant protein

Sequence

MNFQGLVLTD NCKNQWVVGP LIGKGGFGSI YTTNDNNYVV KIEPKANGSL FTEQAFYTRV LKPSVIEEWK KSHNIKHVGL ITCKAFGLYK SINVEYRFLV INRLGADLDA VIRANNNRLP KRSVMLIGIE ILNTIQFMHE QGYSHGDIKA SNIVLDQIDK NKLYLVDYGL VSKFMSNGEH VPFIRNPNKM DNGTLEFTPI DSHKGYVVSR RGDLETLGYC MIRWLGGILP WTKISETKNC ALVSATKQKY VNNTATLLMT SLQYAPRELL QYITMVNSLT YFEEPNYDEF RHILMQGVYY

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

34,274 Da

Sequence Positions

1-300, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for P16913

Species

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of VPK1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VPK1. It may not necessarily be applicable to this product.

NCBI GI #

66275980

NCBI GeneID

3707654

NCBI Accession #

YP_233065.1

UniProt Primary Accession #

P16913

UniProt Secondary Accession #

Q76ZM2

UniProt Related Accession #

AAO89462

GenBank Nucleotide #

NC_006998.1

NCBI Official Full Name

ser/thr kinase

NCBI Official Symbol

B1R

UniProt Protein Name

B1 kinase

UniProt Synonym Protein Names

Serine/threonine-protein kinase 1 (EC:2.7.11.1

UniProt Gene Name

VPK1

NCBI Protein Information

ser/thr kinase

UniProt Comments for VPK1

Essential serine/threonine-protein kinase that plays different role in the viral life cycle (PubMed:1560522, PubMed:1602551). Phosphorylates the host small ribosomal protein RACK1 thereby customizing the ribosomes to a state optimal for viral mRNAs (which contain poly-A leaders) but not for host mRNAs (PubMed:28636603). Facilitates viral DNA replication by inhibiting host BANF1, a cellular host defense responsive to foreign DNA (PubMed:18005698, PubMed:1602551). Phosphorylates host BANF1 on serine and threonine residues; this leads to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity (PubMed:24600006). Indeed, BANF1 activity depends on its DNA-binding property which is blocked by VPK1-mediated phosphorylation (PubMed:18005698). Required for viral intermediate genes expression, probably by inhibiting host BANF1 (PubMed:11287554, PubMed:23891157). Modulates cellular responses via host JUN by two different mechanisms, either by direct phosphorylation or by modulation of upstream JIP1-MAPK complexes (PubMed:16840345). Seems to participates in the accumulation/processing of late proteins and thus in virion maturation (PubMed:26223647).

Research Articles on VPK1

1. trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase, vaccinia virus B1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.