Oxyntomodulin (OXM) ELISA Kit for Mouse

Mouse

Sandwich

Serum, Plasma, Tissue Homogenate, Feces and Urine

3.12pg/ml-100pg/ml.

1.0pg/ml.

No significant cross-reactivity or interference between Mouse OXM and analogues was observed.

Store all reagents at 2-8 degree C

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay CV (%) is less than 15%.

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated OXM concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse OXM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OXM and OXM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OXM in the samples is then determined by comparing the O.D. of the samples to the standard curve.