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Hydrogenase-4 Transcriptional Activator (hyfR) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1026526

MBS1026526 is a recombinant protein targeting hyfR. Also known as Recombinant Escherichia coli Hydrogenase-4 transcriptional activator (hyfR). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1505 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hydrogenase-4 transcriptional activator (hyfR), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Hydrogenase-4 transcriptional activator (hyfR)
Product Gene Name
Sequence
MAMSDEAMFA PPQGITIEAV NGMLAERLAQ KHGKASLLRA FIPLPPPFSP VQLIELHVLK SNFYYRYHDD GSDVTATTEY QGEMVDYSRH AVLLGSSGMA ELRFIRTHGS RFTSQDCTLF NWLARIITPV LQSWLNDEEQ QVALRLLEKD RDHHRVLVDI TNAVLSHLDL DDLIADVARE IHHFFGLASV SMVLGDHRKN EKFSLWCSDL SASHCACLPR CMPGESVLLT QTLQTRQPTL THRADDLFLW QRDPLLLLLA SNGCESALLI PLTFGNHTPG ALLLAHTSST LFSEENCQLL QHIADRIAIA VGNADAWRSM TDLQESLQQE NHQLSEQLLS NLGIGDIIYQ SQAMEDLLQQ VDIVAKSDST VLICGETGTG KEVIARAIHQ LSPRRDKPLV KINCAAIPAS LLESELFGHD KGAFTGAINT HRGRFEIADG GTLFLDEIGD LPLELQPKLL RVLQEREIER LGGSRTIPVN VRVIAATNRD LWQMVEDRQF RSDLFYRLNV FPLELPPLRD RPEDIPLLAK HFTQKMARHM NRAIDAIPTE ALRQLMSWDW PGNVRELENV IERAVLLTRG NSLNLHLNVR QSRLLPTLNE DSALRSSMAQ LLHPTTPEND EEERQRIVQV LRETNGIVAG PRGAATRLGM KRTTLLSRMQ RLGISVREVL
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
75,305 Da
Sequence Positions
1-670, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P71229
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of hyfR recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for hyfR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
hydrogenase-4 transcriptional activator
NCBI Official Symbol
hyfR
NCBI Official Synonym Symbols
ECK2487; JW2476
UniProt Protein Name
DNA-binding transcriptional activator HyfR
UniProt Synonym Protein Names
Hydrogenase-4 transcriptional activator
UniProt Gene Name
hyfR
NCBI Protein Information
hydrogenase-4 transcriptional activator

NCBI Summary for hyfR

HyfR , "hydrogenase four regulator," controls the expression of genes responsible for the proton-translocating formate hydrogenase system and for formate transport under anaerobiosis, low pH, and post-exponential growth conditions . [More information is available at EcoCyc: G7308].

UniProt Comments for hyfR

A transcriptional activator of its own operon; when overexpressed operon expression is strongly enhanced by low pH (under pH 6.0), strongly inhibited by O2 but only weakly stimulated by fumarate (PubMed:12426353, PubMed:14702328). Expression in situ is very weak (PubMed:12426353, PubMed:14702328).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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