(Rhesus Macaque) TNF Receptor-Associated Factor 6 (TRAF6) Macaca Mulatta Recombinant Protein

Function: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Mediates activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation

By similarity.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Homotrimer

By similarity. Homooligomer

By similarity. N-terminal region is dimeric while C-terminal region is trimeric; maybe providing a mode of oligomerization. Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, TRAF, TRAF-interacting protein TRIP and TNF receptor associated protein TDP2. Interacts with IL17R. Interacts with SQSTM1 bridging NTRK1 and NGFR. Forms a ternary complex with SQSTM1 and PRKCZ. Interacts with PELI1, PELI2 and PELI3. Binds UBE2V1. Interacts with MAVS/IPS1. Interacts with TAX1BP1. Interacts with IL1RL1. Interacts with TRAFD1. Interacts with ZNF675. Interacts with AJUBA. Interacts with TICAM1 and TICAM2. Interacts with ZFAND5. Interacts with ARRB1 and ARRB2. Interacts with MAP3K7 and TAB1/MAP3K7IP1; during IL-1 signaling. Interacts with UBE2N. Interacts with TGFBR1, HDAC1 and RANGAP1. Interacts with AKT1, AKT2 and AKT3. Interacts (via TRAF domains) with NUMBL (via C-terminal). Interacts (via TRAF domains) with WDR34 (via WD domains). Interacts with RBCK1

By similarity. Interacts with TRAF3IP2

By similarity. Interacts with LIMD1 (via LIM domains)

By similarity. Interacts with RSAD2/viperin

By similarity. Interacts with IFIT3 (via N-terminus)

By similarity.

Subcellular location: Cytoplasm

By similarity. Cytoplasm › cell cortex

By similarity. Nucleus

By similarity. Lipid droplet

By similarity. Note: RSAD2/viperin recruits it to the lipid droplet

By similarity.

Domain: The coiled coil domain mediates homo- and hetero-oligomerization

By similarity.The MATH/TRAF domain binds to receptor cytoplasmic domains

By similarity.

Post-translational modification: Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1

By similarity.Polyubiquitinated; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form

By similarity.

Sequence similarities: Belongs to the TNF receptor-associated factor family. A subfamily.Contains 1 MATH domain.Contains 1 RING-type zinc finger.Contains 2 TRAF-type zinc fingers.