Beta-1,3-N-Acetylglucosaminyltransferase Radical Fringe (RFNG) Chicken Recombinant Protein

Function: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Plays an important role in limb outgrowth, it directs the formation and positioning of the apical ectodermal ridge (AER), one of the key organizer centers of vertebrate limb development.

Catalytic activity: Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactor: Manganese

By similarity.

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein

Potential.

Developmental stage: Detected at stage 15 in the presumptive dorsal-limb ectoderm. At stages 16-18 expression is restricted to the dorsal side with higher concentration at the dorsoventral boundary and later in the AER. Expression remains restricted to the dorsal ectoderm and the AER until stages 22-24 when it starts to fade in the dorsal ectoderm, but remains in the AER until the last stage examined (27).

Sequence similarities: Belongs to the glycosyltransferase 31 family.