Arabidopsis Thaliana COP1 Recombinant Protein – Cat# MBS1035956

E3 Ubiquitin-Protein Ligase COP1 (COP1) Arabidopsis Thaliana Recombinant Protein

Name: COP1 Recombinant Protein; Arabidopsis Thaliana; MyBioSource
Brand: MyBioSource
SKU: MBS1035956
Price: 1040 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1035956

MBS1035956 is a recombinant protein targeting COP1. Also known as Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1 (COP1) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1040 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

E3 ubiquitin-protein ligase COP1 (COP1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase COP1 (COP1) , partial

Product Gene Name

COP1 recombinant protein

Sequence

TFTRYSRLRV IAEIRHGDIF HSANIVSSIE FDRDDELFAT AGVSRCIKVF DFSSVVNEPA DMQCPIVEMS TRSKLSCLSW NKHEKNHIAS SDYEGIVTVW DVTTRQSLME YEEHEKRAWS VDFSRTEPSM LVSGSDDCKV KVWCTRQEAS VINIDMKANI CCVKYNPGSS NYIAVGSADH HIHYYDLRNI SQPLHVFSGH KKAVSYVKFL SNNELASAST DSTLRLWDVK DNLPVRTFRG HTNEKNFVGL TVNSEYLACG SETNEVYVYH KEITRPVTSH RFGSPDMDDA EEEAGSYFIS AVCWKSDSPT MLTANSQGTI KVLVLAA

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

76,188 Da

Sequence Positions

349-675aa; Partial

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for P43254

Species

Arabidopsis thaliana (Mouse-ear cress)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of COP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COP1. It may not necessarily be applicable to this product.

NCBI GI #

15225760

NCBI GeneID

817857

NCBI Accession #

NP_180854.1

UniProt Primary Accession #

P43254

UniProt Secondary Accession #

O23974; O23975; O48770; Q1JPL6

UniProt Related Accession #

P43254

GenBank Nucleotide #

NM_128855.4

NCBI Official Full Name

Transducin/WD40 repeat-like superfamily protein

NCBI Official Symbol

COP1

NCBI Official Synonym Symbols

ARABIDOPSIS THALIANA CONSTITUTIVE PHOTOMORPHOGENIC 1; ATCOP1; CONSTITUTIVE PHOTOMORPHOGENIC 1; DEETIOLATED MUTANT 340; DET340; EMB168; EMBRYO DEFECTIVE 168; FUS1; FUSCA 1; T21L14.11; T21L14_11

UniProt Protein Name

E3 ubiquitin-protein ligase COP1

UniProt Synonym Protein Names

Constitutive photomorphogenesis protein 1

Protein Family

Cruxrhodopsin

UniProt Gene Name

COP1

NCBI Protein Information

Transducin/WD40 repeat-like superfamily protein

NCBI Summary for COP1

Represses photomorphogenesis and induces skotomorphogenesis in the dark. Contains a ring finger zinc-binding motif, a coiled-coil domain, and several WD-40 repeats, similar to G-beta proteins. The C-terminus has homology to TAFII80, a subunit of the TFIID component of the RNA polymerase II of Drosophila. Nuclear localization in the dark and cytoplasmic in the light.

UniProt Comments for COP1

E3 ubiquitin-protein ligase that acts as a repressor of photomorphogenesis and as an activator of etiolation in darkness. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Represses photomorphogenesis in darkness by mediating ubiquitination and subsequent proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Down-regulates MYB21, probably via ubiquitination process. Light stimuli abrogate the repression of photomorphogenesis, possibly due to its localization to the cytoplasm. Could play a role in switching between skotomorphogenetic and photomorphogenetic pathways. Mediates the ubiquitination-dependent degradation of HY5 in the darkness during seedling development (e.g. hypocotyl growth) (PubMed:26474641). Represses CIP7 in darkness (PubMed:9668129).

Research Articles on COP1

1. ABI4 and HY5 antagonistically regulate the expression of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and the subsequent greening process. In turn, ABI4 and HY5 are targeted for degradation by COP1 in the light and dark, respectively, to ensure a proper interplay of ABI4 and HY5 actions during seedling de-etiolation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with COP1 recombinant protein

Reference Product	PubMed Publications
HY5 recombinant protein	>59 publications with COP1 and HY5
CRY2 recombinant protein	>15 publications with COP1 and CRY2
HFR1 recombinant protein	>9 publications with COP1 and HFR1
SPA4 recombinant protein	>7 publications with COP1 and SPA4
SPA3 recombinant protein	>6 publications with COP1 and SPA3

Pathways associated with COP1 recombinant protein

Products by Pathway	Pathway Diagram
Autodegradation Of The E3 Ubiquitin Ligase COP1 Pathway antibodies	Autodegradation Of The E3 Ubiquitin Ligase COP1 Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
G1/S DNA Damage Checkpoints Pathway antibodies	G1/S DNA Damage Checkpoints Pathway Diagram
Stabilization Of P53 Pathway antibodies	Stabilization Of P53 Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
P53-Dependent G1 DNA Damage Response Pathway antibodies	P53-Dependent G1 DNA Damage Response Pathway Diagram
P53-Dependent G1/S DNA Damage Checkpoint Pathway antibodies	P53-Dependent G1/S DNA Damage Checkpoint Pathway Diagram

Organs/Tissues associated with COP1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with COP1 and Bone
Connective Tissue Antibodies	>4 publications with COP1 and Connective Tissue
Heart Antibodies	>3 publications with COP1 and Heart
Muscle Antibodies	>3 publications with COP1 and Muscle
Brain Antibodies	>3 publications with COP1 and Brain
Vascular Antibodies	>2 publications with COP1 and Vascular
Embryonic Tissue Antibodies	>2 publications with COP1 and Embryonic Tissue
Ear Antibodies	>1 publications with COP1 and Ear
Kidney Antibodies	>1 publications with COP1 and Kidney
Umbilical Cord Antibodies	>1 publications with COP1 and Umbilical Cord