Probable 4-Amino-4-Deoxy-L-Arabinose-Phosphoundecaprenol Flippase Subunit Arnf (arnF) Escherichia Coli Recombinant Protein

Involved in the modification of lipid A phosphates with aminoarabinose that is required for polymyxin B and cationic antimicrobial peptide resistance. [More information is available at EcoGene: EG14094]. ArnF is an integral membrane component of the ArnE/ArnF undecaprenyl-phosphate--L-Ara4N flippase. [More information is available at EcoCyc: G7171].

Function: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the periplasmic side of the inner membrane. Ref.6

Pathway: Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP-Rule MF_00538

Subunit structure: Heterodimer of ArnE and ArnF

Probable. Ref.6

Subcellular location: Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_00538.

Induction: Induced by BasR. Ref.5

Disruption phenotype: Cells lacking this gene are polymyxin sensitive. Lipid A is no longer modified with L-Ara4N even though the level of the lipid-linked donor of the L-Ara4N moiety, alpha-L-Ara4N-phosphoundecaprenol, is not reduced. However, the alpha-L-Ara4N-phosphoundecaprenol is less concentrated on the periplasmic surface of the inner membrane when compared to wild type. Ref.6

Sequence similarities: Belongs to the ArnF family.

Sequence caution: The sequence AAB04894.1 differs from that shown. Reason: Frameshift at position 128.