15-Deoxy-Prostaglandin J2 (15d-PGJ2) ELISA Kit for Mouse

Principle of the Assay: 15D-PGJ2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-15D-PGJ2 antibody and an 15D-PGJ2-HRP conjugate. The assay sample and buffer are incubated together with 15D-PGJ2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 15D-PGJ2 concentration since 15D-PGJ2 from samples and 15D-PGJ2-HRP conjugate compete for the anti-15D-PGJ2 antibody binding site. Since the number of sites is limited, as more sites are occupied by 15D-PGJ2 from the sample, fewer sites are left to bind 15D-PGJ2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 15D-PGJ2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This 15D-PGJ2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse 15D-PGJ2. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of 15d-PGJ2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS739421 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 15-deoxy-Prostaglandin J2 (15d-PGJ2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 15d-PGJ2. The ELISA analytical biochemical technique of the MBS739421 kit is based on 15d-PGJ2 antibody-15d-PGJ2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 15d-PGJ2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 15d-PGJ2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).