• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DNA-Directed RNA Polymerase I Subunit RPA1 (POLR1A) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1056774

MBS1056774 is a recombinant protein targeting POLR1A. Also known as Recombinant Human DNA-directed RNA polymerase I subunit RPA1 (POLR1A) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$990 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA-directed RNA polymerase I subunit RPA1 (POLR1A), Recombinant Protein
Full Product Name
Recombinant Human DNA-directed RNA polymerase I subunit RPA1 (POLR1A) , partial
Product Gene Name
POLR1A recombinant protein
Sequence
LKWQRSLCEP GEAVGLLAAQ SIGEPSTQMT LNTFHFAGRG EMNVTLGIPR LREILMVASA NIKTPMMSVP VLNTKKALKR VKSLKKQLTR VCLGEVLQKI DVQESFCMEE KQNKFQVYQL RFQFLPHAYY QQEKCLRPED ILRFMETRFF KLLMESIKKK NNKASAFRNV NTRRATQRDL DNAGELGRSR GEQEGDEEEE GHIVDAEAEE GDADASDAKR KEKQEEEVDY ESEEEEEREG EENDDEDMQE ERNPHREGAR KTQEQDEEVG LGTEEDPSLP ALLTQPRKPT HSQEPQGPEA
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
194,811 Da
Sequence Positions
1198-1497aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
616404
3D Structure
ModBase 3D Structure for O95602
Species
Human
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of POLR1A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR1A. It may not necessarily be applicable to this product.

NCBI GI #
103471997
NCBI GeneID
25885
NCBI Accession #
NP_056240.2
UniProt Primary Accession #
O95602
UniProt Secondary Accession #
Q0VG05; Q9UEH0; Q9UFT9; B7Z7T0; D6W5M0
UniProt Related Accession #
O95602
GenBank Nucleotide #
NM_015425.5
NCBI Official Full Name
DNA-directed RNA polymerase I subunit RPA1
NCBI Official Synonym Full Names
RNA polymerase I subunit A
NCBI Official Symbol
POLR1A
NCBI Official Synonym Symbols
A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA1
UniProt Synonym Protein Names
A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit; RPA194
UniProt Gene Name
POLR1A
UniProt Synonym Gene Names
RNA polymerase I subunit A1; RPA194
NCBI Protein Information
DNA-directed RNA polymerase I subunit RPA1

NCBI Summary for POLR1A

The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]
Show more

UniProt Comments for POLR1A

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition ().
Show more

Research Articles on POLR1A

1. Data show that BRCA1 interacts directly with the basal RNA polymerase I (Pol-I) transcription factors as well as interacting with RNA Pol-I itself.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with POLR1A recombinant protein

 Products by Pathway  Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Negative Epigenetic Regulation Of RRNA Expression Pathway antibodies  Negative Epigenetic Regulation Of RRNA Expression Pathway Diagram
 NoRC Negatively Regulates RRNA Expression Pathway antibodies  NoRC Negatively Regulates RRNA Expression Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram

Diseases associated with POLR1A recombinant protein

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with POLR1A and Disease Models, Animal

Organs/Tissues associated with POLR1A recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with POLR1A and Liver
 Embryonic Tissue Antibodies  >1 publications with POLR1A and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000