Aquaporin PIP2-1 (PIP2-1) (PIP2A) Arabidopsis Thaliana Recombinant Protein

a member of the plasma membrane intrinsic protein subfamily PIP2. localizes to the plasma membrane and exhibits water transport activity in Xenopus oocyte. expressed specifically in the vascular bundles and protein level increases slightly during leaf dev. When expressed in yeast cells can conduct hydrogen peroxide into those cells.

Function: Water channel required to facilitate the transport of water across cell membrane. Probably involved in root water uptake. Its function is impaired by Hg2+. Ref.1

Subcellular location: Cell membrane; Multi-pass membrane protein. Note: A fuzzy intracellular localization is induced by salt (NaCl) treatment. Ref.7 Ref.11

Tissue specificity: Predominantly expressed in roots and green siliques. Also expressed at lower level above ground and in flower buds. Ref.1 Ref.8

Domain: Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Post-translational modification: Ubiquitinated by RMA1, leading to proteasomal degradation. Ref.12The phosphorylation at Ser-280 and Ser-283 is altered by salt (NaCl) and hydrogen peroxide H2O2 treatments. Phosphorylation of Ser-283 is required for plasma membrane targeting.

Sequence similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily. [View classification]