• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NADH-Cytochrome B5 Reductase 2 (MCR1) Saccharomyces Cerevisiae Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1070482

MBS1070482 is a recombinant protein targeting MCR1. Also known as Recombinant Saccharomyces cerevisiae NADH-cytochrome b5 reductase 2 (MCR1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Please Inquire
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NADH-cytochrome b5 reductase 2 (MCR1), Recombinant Protein
Full Product Name
Recombinant Saccharomyces cerevisiae NADH-cytochrome b5 reductase 2 (MCR1)
Product Gene Name
MCR1 recombinant protein
Product Synonym Gene Name
MCR1
Product Synonym Names
Recombinant NADH-cytochrome b5 reductase 2 (MCR1); NADH-cytochrome b5 reductase 2 EC= 1.6.2.2; Mitochondrial cytochrome b reductase p34/p32 Cleaved into the following 2 chains: 1. NADH-cytochrome b5 reductase p34 form 2. NADH-cytochrome b5 reductase p32 f
Sequence
ESNKVFKGDD KWIDLPISKI EEESHDTRRF TFKLPTEDSE MGLVLASALF AKFVTPKGSN VVRPYTPVSD LSQKGHFQLV VKHYEGGKMT SHLFGLKPND TVSFKGPIMK WKWQPNQFKS ITLLGAGTGI NPLYQLAHHI VENPNDKTKV NLLYGNKTPQ DILLRKELDA LKEKYPDKFN VTYFVDDKQD DQDFDGEISF ISKDFIQEHV PGPKESTHLF VCGPPPFMNA YSGEKKSPKD QGELIGILNN LGYSKDQVFK F
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
34,138 Da
Sequence Positions
42-302
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
Chromosome: XI; NC_001143.9 (166544..167452). Location: chromosome: XI
3D Structure
ModBase 3D Structure for P36060
Species
Saccharomyces cerevisiae (strain 204508 / S288c) (Baker's yeast)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MCR1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCR1. It may not necessarily be applicable to this product.

NCBI GI #
6322699
NCBI GeneID
853707
NCBI Accession #
NP_012772.1
UniProt Primary Accession #
P36060
UniProt Secondary Accession #
A2TBM9; D6VX47
UniProt Related Accession #
P36060
GenBank Nucleotide #
NM_001179716.1
NCBI Official Full Name
Mcr1p
NCBI Official Symbol
MCR1
UniProt Protein Name
NADH-cytochrome b5 reductase 2
UniProt Synonym Protein Names
Mitochondrial cytochrome b reductase; p34/p32
Protein Family
Probable phosphatidylethanolamine transferase
UniProt Gene Name
MCR1
UniProt Entry Name
MCR1_YEAST
NCBI Protein Information
Mcr1p

UniProt Comments for MCR1

Function: The outer membrane form may mediate the reduction of outer membrane cytochrome b5, and the soluble inter-membrane space form may transfer electrons from external NADH to cytochrome c, thereby mediating an antimycin-insensitive, energy-coupled oxidation of external NADH by yeast mitochondria. Involved in the reduction of D-erythroascorbyl free radicals. Ref.9

Catalytic activity: NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactor: FAD.

Subcellular location: NADH-cytochrome b5 reductase p32 form: Mitochondrion intermembrane space Ref.6 Ref.10 Ref.13. NADH-cytochrome b5 reductase p34 form: Mitochondrion outer membrane; Single-pass membrane protein Ref.6 Ref.10 Ref.13.

Induction: By osmotic stress. Ref.12

Post-translational modification: There are two isoforms of NADH-cytochrome b5 reductase, a 34 kDa form (p34) and a 32 kDa form (p32). The p34 form becomes firmly anchored to the outer mitochondrial membrane after an incomplete translocation arrest. The p32 form is formed after translocation of the p34 precursor to the inner mitochondrial membrane, where it is processed by mitochondrial inner membrane peptidase (IMP) complex and released to the intermembrane space.

Miscellaneous: Present with 1920 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Contains 1 FAD-binding FR-type domain.

Mass spectrometry: Molecular mass is 34137 Da from positions 1 - 302. Ref.6
Show more

Research Articles on MCR1

1. The import pathways of the two MCR1 forms diverge in a stage before the encounter with the TOM complex and their mechanism of biogenesis represents a unique example how to achieve dual localization within one organelle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MCR1 recombinant protein

 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Biosynthesis Of Secondary Metabolites Pathway antibodies  Biosynthesis Of Secondary Metabolites Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000