Escherichia Coli eptB Recombinant Protein – Cat# MBS1071420

Phosphoethanolamine Transferase Eptb (eptB) Escherichia Coli Recombinant Protein

Name: eptB Recombinant Protein; Escherichia Coli; MyBioSource
Brand: MyBioSource
SKU: MBS1071420
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1071420

MBS1071420 is a recombinant protein targeting eptB. Also known as Recombinant Escherichia coli Phosphoethanolamine transferase eptB (eptB). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phosphoethanolamine transferase eptB (eptB), Recombinant Protein

Full Product Name

Recombinant Escherichia coli Phosphoethanolamine transferase eptB (eptB)

Product Gene Name

eptB recombinant protein

Product Synonym Gene Name

eptB; yhjW

Product Synonym Names

Recombinant Phosphoethanolamine transferase eptB (eptB); Phosphoethanolamine transferase eptB EC= 2.7.-.-

Sequence

MRYIKSITQQ KLSFLLAIYI GLFMNGAVFY RRFGSYAHDF TVWKGISAVV ELAATVLVTF FLLRLLSLFG RRSWRILASL VVLFSAGASY YMTFLNVVIG YGIIASVMTT DIDLSKEVVG LNFILWLIAV SALPLILIWN NRCRYTLLRQ LRTPGQRIRS LAVVVLAGIM VWAPIRLLDI QQKKVERATG VDLPSYGGVV ANSYLPSNWL SALGLYAWAR VDESSDNNSL LNPAKKFTYQ APQNVDDTYV VFIIGETTRW DHMGIFGYER NTTPKLAQEK NLAAFRGYSC DTATKLSLRC MFVRQGGAED NPQRTLKEQN IFAVLKQLGF SSDLYAMQSE MWFYSNTMAD NIAYREQIGA EPRNRGKPVD DMLLVDEMQQ SLGRNPDGKH LIILHTKGSH FNYTQRYPRS FAQWKPECIG VDSGCTKAQM INSYDNSVTY VDHFISSVID QVRDKKAIVF YAADHGESIN EREHLHGTPR ELAPPEQFRV PMMVWMSDKY LENPANAQAF AQLKKEADMK VPRRHVELYD TIMGCLGYTS PDGGINENNN WCHIPQAKEA AAN

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

63,804 Da

Sequence Positions

1-563

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

NC_000913.2 (3706807..3708498, complement)

3D Structure

ModBase 3D Structure for P37661

Species

Escherichia coli (strain K12)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of eptB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for eptB. It may not necessarily be applicable to this product.

NCBI GI #

90111611

NCBI GeneID

948068

NCBI Accession #

NP_418002.2

UniProt Primary Accession #

P37661

UniProt Secondary Accession #

Q2M7K7

GenBank Nucleotide #

NC_000913.2

NCBI Official Full Name

KDO phosphoethanolamine transferase, Ca(2+)-inducible

NCBI Official Symbol

eptB

NCBI Official Synonym Symbols

ECK3533; JW5660; pagC; yhjW

UniProt Protein Name

Phosphoethanolamine transferase EptB

Protein Family

Kdo(2)-lipid A phosphoethanolamine 7''-transferase

UniProt Gene Name

eptB

UniProt Synonym Gene Names

yhjW

UniProt Entry Name

EPTB_ECOLI

NCBI Protein Information

KDO phosphoethanolamine transferase, Ca(2+)-inducible

NCBI Summary for eptB

Localized to the inner membrane by subcellular fractionation (Reynolds, 2005). [More information is available at EcoGene: EG12267]. The eptB gene encodes the Ca2+-induced phosphoethanolamine transferase . [More information is available at EcoCyc: EG12267].

UniProt Comments for eptB

Function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases diacylglycerol. Ref.5

Cofactor: Calcium. Ref.5

Enzyme regulation: Inhibited by calcium concentrations higher than 1 mM. Ref.5

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.5.

Sequence similarities: Belongs to the phosphoethanolamine transferase family. EptB subfamily.

Research Articles on eptB

1. the active site of EptB faces the periplasmic surface of the inner membrane

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.