Sensor Histidine Kinase Dcus (dcuS) Escherichia Coli Recombinant Protein

Function: Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation.

Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulation: Autophosphorylation is stimulated by the presence of C4-dicarboxylates such as fumarate, succinate, malate, and tartrate.

Subunit structure: Homodimer

Probable. Ref.9 Ref.10

Subcellular location: Cell inner membrane; Multi-pass membrane protein.

Domain: The periplasmic domain is involved in C4-dicarboxylate binding and sensing. The structural disorder in the cytoplasmic PAS domain has an important role in signal transduction to the kinase domain and may be the decisive structural feature that characterizes the activated kinase. Ref.8 Ref.9 Ref.10

Post-translational modification: Autophosphorylated. The phosphoryl group is rapidly transferred to DcuR.

Miscellaneous: The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear.

Sequence similarities: Contains 1 histidine kinase domain.Contains 1 PAS (PER-ARNT-SIM) domain.