Nickel-Responsive Regulator (nikR) Escherichia Coli (Strain K12) Recombinant Protein

Transcriptional repressor of the nikABCDE operon. Is active in the presence of excessive concentrations of intracellular nickel.

Isolation and characterization of the nikR gene encoding a nickel-responsive regulator in Escherichia coli.De Pina K., Desjardin V., Mandrand-Berthelot M.-A., Giordano G., Wu L.-F.J. Bacteriol. 181:670-674(1999) Rhs elements of Escherichia coli K-12 complex composites of shared and unique components that have different evolutionary histories.Zhao S., Sandt C.H., Feulner G., Vlazny D.A., Gray J.A., Hill C.W.J. Bacteriol. 175:2799-2808(1993) Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.Nucleic Acids Res. 22:2576-2586(1994) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Analysis of products of the Escherichia coli genomic genes and regulation of their expressions an applicable procedure for genomic analysis of other microorganisms.Talukder A.A., Yanai S., Yamada M.Biosci. Biotechnol. Biochem. 58:117-120(1994) NikR is a ribbon-helix-helix DNA-binding protein.Chivers P.T., Sauer R.T.Protein Sci. 8:2494-2500(1999) Regulation of high affinity nickel uptake in bacteria. Ni2+-dependent interaction of NikR with wild-type and mutant operator sites.Chivers P.T., Sauer R.T.J. Biol. Chem. 275:19735-19741(2000) Crystal structure of the nickel-responsive transcription factor NikR.Schreiter E.R., Sintchak M.D., Guo Y., Chivers P.T., Sauer R.T., Drennan C.L.Nat. Struct. Biol. 10:794-799(2003)

Small volumes of nikR recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1081205 is a recombinant protein targeting nikR. Also known as Recombinant Escherichia coli (strain K12) Nickel-responsive regulator. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.