Human PACAP38 (Pituitary Adenylate Cyclase-Activating Polypeptide 38) ELISA Kit

This human PACAP38 ELISA kit uses a sandwich immunoassay format to measure pituitary adenylate cyclase-activating polypeptide 38 concentrations in serum, plasma, and cell culture supernatants, supporting research into trigeminal nociception, migraine pathophysiology, hypothalamic-pituitary axis regulation, and neuropeptide signaling.

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of PACAP38 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

Small volumes of PACAP38 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS109020 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pituitary Adenylate Cyclase Activating Polypeptide 38 (PACAP38) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PACAP38. The ELISA analytical biochemical technique of the MBS109020 kit is based on PACAP38 antibody-PACAP38 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PACAP38 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PACAP38. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).