Leptospira IgM (LS-IgM) ELISA Kit for Human

Intended Uses: For the qualitative determination of activated Human Leptospira IgM (LS-IgM) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.

Principle of the Assay||The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human LS-IgM in samples. Add sample to wells pre-coated with one Human Leptospira IgM antigen, at same time add HRP-conjugated Human Leptospira IgM antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human LS-IgM in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.

Small volumes of LS-IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS109034 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leptospira IgM (LS-IgM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LS-IgM. The ELISA analytical biochemical technique of the MBS109034 kit is based on LS-IgM antibody-LS-IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LS-IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LS-IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).