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Guanidinoacetate N-Methyltransferase (GAMT) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS109572

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used to determine the level of GAMT (hereafter termed "analyte") in undiluted original Mouse body fluids and tissue homogenates.… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Guanidinoacetate N-Methyltransferase (GAMT), ELISA Kit
Full Product Name
Rat Guanidinoacetate N-Methyltransferase (GAMT) ELISA Kit
Product Gene Name
GAMT elisa kit
Species Reactivity
Rat
Assay Type
Quantitative Sandwich
Samples
Mouse Body Fluids And Tissue Homogenates
Detection Range
0.625ng/ml-20ng/ml
Sensitivity
0.1ng/ml.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (?) are less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) are less than 15%
Molecular Weight
29,377 Da

Other Notes

Small volumes of GAMT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAMT purchase

MBS109572 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Guanidinoacetate N-Methyltransferase (GAMT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAMT. The ELISA analytical biochemical technique of the MBS109572 kit is based on GAMT antibody-GAMT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAMT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAMT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GAMT. It may not necessarily be applicable to this product.

NCBI GI #
6014689
NCBI GeneID
2593
NCBI Accession #
AAF01461.1
UniProt Secondary Accession #
Q53Y34; Q8WVJ1; A8K0A0
UniProt Related Accession #
Q14353
NCBI Official Full Name
guanidinoacetate N-methyltransferase
NCBI Official Synonym Full Names
guanidinoacetate N-methyltransferase
NCBI Official Symbol
GAMT
NCBI Official Synonym Symbols
PIG2; CCDS2; TP53I2; HEL-S-20
UniProt Protein Name
Guanidinoacetate N-methyltransferase
Protein Family
Guanidinoacetate N-methyltransferase
UniProt Gene Name
GAMT
NCBI Protein Information
guanidinoacetate N-methyltransferase

NCBI Summary for GAMT

The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. Pseudogenes of this gene are found on chromosomes 2 and 13. [provided by RefSeq, Feb 2012]
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UniProt Comments for GAMT

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor (PubMed:26003046, PubMed:24415674, PubMed:26319512). Important in nervous system development (PubMed:24415674).

Research Articles on GAMT

1. Measurements of creatine and guanidinoacetate in plasma are recommended for the diagnosis of AGAT and GAMT deficiency.Definitive confirmation of the diagnosis requires DNA sequencing of the appropriate gene and (if molecular analysis is ambiguous) measurement of AGAT or GAMT enzyme activity or of CRTR-mediated transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GAMT elisa kit

 Reference Product  PubMed Publications
 GATM elisa kit  >4 publications with GAMT and GATM
 CKB elisa kit  >1 publications with GAMT and CKB

Pathways associated with GAMT elisa kit

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Creatine Metabolism Pathway antibodies  Creatine Metabolism Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with GAMT elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >53 publications with GAMT and Nervous System Diseases
 Brain Diseases Antibodies  >42 publications with GAMT and Brain Diseases
 Guanidinoacetate methyltransferase deficiency Antibodies  >34 publications with GAMT and Guanidinoacetate methyltransferase deficiency
 Seizures Antibodies  >23 publications with GAMT and Seizures
 Intellectual Disability Antibodies  >21 publications with GAMT and Intellectual Disability
 Amino Acid Metabolism, Inborn Errors Antibodies  >17 publications with GAMT and Amino Acid Metabolism, Inborn Errors
 Heart Diseases Antibodies  >6 publications with GAMT and Heart Diseases
 Liver Diseases Antibodies  >6 publications with GAMT and Liver Diseases
 Muscular Diseases Antibodies  >5 publications with GAMT and Muscular Diseases
 Learning Disorders Antibodies  >5 publications with GAMT and Learning Disorders

Organs/Tissues associated with GAMT elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >80 publications with GAMT and Brain
 Blood Antibodies  >57 publications with GAMT and Blood
 Liver Antibodies  >43 publications with GAMT and Liver
 Muscle Antibodies  >25 publications with GAMT and Muscle
 Heart Antibodies  >15 publications with GAMT and Heart
 Kidney Antibodies  >14 publications with GAMT and Kidney
 Pancreas Antibodies  >8 publications with GAMT and Pancreas
 Embryonic Tissue Antibodies  >5 publications with GAMT and Embryonic Tissue
 Testis Antibodies  >3 publications with GAMT and Testis
 Eye Antibodies  >3 publications with GAMT and Eye
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