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Biofilm PGA Synthesis Protein Pgad (pgaD) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1096255

MBS1096255 is a recombinant protein targeting pgaD. Also known as Recombinant Escherichia coli Biofilm PGA synthesis protein PgaD (pgaD). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Biofilm PGA synthesis protein PgaD (pgaD), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Biofilm PGA synthesis protein PgaD (pgaD)
Product Gene Name
Product Synonym Gene Name
pgaD; ycdP
Product Synonym Names
Recombinant Biofilm PGA synthesis protein PgaD (pgaD); Biofilm PGA synthesis protein PgaD
Sequence
MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA RSRLQFYFLL AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA IPDELYQQLQ KSHRMSVHFT SQGQIKMVVS EKALVRA
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
16,082 Da
Sequence Positions
1-137
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
NC_000913.2 (1085329..1085742, complement)
3D Structure
ModBase 3D Structure for P69432
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of pgaD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for pgaD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
required for biofilm adhesin polysaccharide PGA synthesis
NCBI Official Symbol
pgaD
NCBI Official Synonym Symbols
ECK1011; hmsS; JW1006; ycdP
UniProt Protein Name
Biofilm PGA synthesis protein PgaD
UniProt Gene Name
pgaD
UniProt Synonym Gene Names
ycdP
UniProt Entry Name
PGAD_ECOLI
NCBI Protein Information
required for biofilm adhesin polysaccharide PGA synthesis

NCBI Summary for pgaD

PgaD complements a mutation in the homologous Y. pestis hemin storage gene hmsS. [More information is available at EcoGene: EG13862]. The PgaD protein is involved in biofilm formation. [More information is available at EcoCyc: G6528].

UniProt Comments for pgaD

Function: Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA. Ref.4 Ref.6 Ref.7

Subcellular location: Cell inner membrane; Multi-pass membrane protein.

Induction: Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD. Ref.7

Disruption phenotype: Cells lacking this gene do not synthesize PGA. Ref.4 Ref.6
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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