Holliday Junction ATP-Dependent DNA Helicase Ruva (ruvA) Escherichia Coli (Strain K12) Recombinant Protein

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.

Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes.Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.Nucleic Acids Res. 16:1541-1549(1988) Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination.Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.J. Bacteriol. 170:4322-4329(1988) A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:379-392(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease.Takahagi M., Iwasaki H., Nakata A., Shinagawa H.J. Bacteriol. 173:5747-5753(1991) RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro.Tsaneva I.R., Mueller B., West S.C.Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993) A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair.Babu M., Beloglazova N., Flick R., Graham C., Skarina T., Nocek B., Gagarinova A., Pogoutse O., Brown G., Binkowski A., Phanse S., Joachimiak A., Koonin E.V., Savchenko A., Emili A., Greenblatt J., Edwards A.M., Yakunin A.F.Mol. Microbiol. 79:484-502(2011) Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction.Rafferty J.B., Sedelnikova S.E., Hargreaves D., Artymiuk P.J., Baker P.J., Sharples G.J., Mahdi A.A., Lloyd R.G., Rice D.W.Science 274:415-421(1996) Crystal structure of E Coli RuvA with bound DNA Holliday junction at 6-A resolution.Hargreaves D., Rice D.W., Sedelnikova S.E., Artymiuk P.J., Lloyd R.G., Rafferty J.B.Nat. Struct. Biol. 5:441-446(1998) Functional analyses of the domain structure in the Holliday junction binding protein RuvA.Nishino T., Ariyoshi M., Iwasaki H., Shinagawa H., Morikawa K.Structure 6:11-21(1998) Modulation of RuvB function by the mobile domain III of the Holliday junction recognition protein RuvA.Nishino T., Iwasaki H., Kataoka M., Ariyoshi M., Fujita T., Shinagawa H., Morikawa K.J. Mol. Biol. 298:407-416(2000) Processing of recombination intermediates by the RuvABC proteins.West S.C.Annu. Rev. Genet. 31:213-244(1997)

Small volumes of ruvA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1102272 is a recombinant protein targeting ruvA. Also known as Recombinant Escherichia coli (strain K12) Holliday junction ATP-dependent DNA helicase RuvA. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.