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PTS System Mannitol-Specific Cryptic EIICB Component (cmtA) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1105832

MBS1105832 is a recombinant protein targeting cmtA. Also known as Recombinant Escherichia coli PTS system mannitol-specific cryptic EIICB component (cmtA). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PTS system mannitol-specific cryptic EIICB component (cmtA), Recombinant Protein
Full Product Name
Recombinant Escherichia coli PTS system mannitol-specific cryptic EIICB component (cmtA)
Product Gene Name
cmtA recombinant protein
Product Synonym Gene Name
cmtA
Product Synonym Names
Recombinant PTS system mannitol-specific cryptic EIICB component (cmtA); PTS system mannitol-specific cryptic EIICB component; EIICB-Mtl; EII-Mtl Including the following 2 domains: Mannitol permease IIC component; PTS system mannitol-specific EIIC compone
Sequence
MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA KIVGPMITYL LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG SMIMGPLGGL VIKYVDKALE KRIPAGFEMV INNFSLGIAG MLLCLLGFEV IGPAVLIANT FVKECIEALV HAGYLPLLSV INEPAKVLFL NNAIDQGVYY PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS KRSAPGAMII HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA QSANAVKAMK QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL AIEVKHYAIE NVPADADIVV THASLEGRVK RVTDKPLILI NNYIGDPKLD TLFNQLTAEH KH
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
48,971 Da
Sequence Positions
1-462
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
NC_000913.2 (3075493..3076881, complement)
3D Structure
ModBase 3D Structure for P69826
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of cmtA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for cmtA. It may not necessarily be applicable to this product.

NCBI GI #
16130834
NCBI GeneID
945256
NCBI Accession #
NP_417408.1
UniProt Primary Accession #
P69826
UniProt Secondary Accession #
P32059; Q2M9R0
GenBank Nucleotide #
NC_000913.2
NCBI Official Full Name
predicted fused mannitol-specific PTS enzymes: IIB component/IIC component
NCBI Official Symbol
cmtA
NCBI Official Synonym Symbols
ECK2928; JW2900
UniProt Protein Name
PTS system mannitol-specific cryptic EIICB component
UniProt Synonym Protein Names
EIICB-Mtl; EII-Mtl
Protein Family
PTS system mannitol-specific cryptic EIICB component
UniProt Gene Name
cmtA
UniProt Synonym Gene Names
EII-Mtl
UniProt Entry Name
PTMCB_ECOLI
NCBI Protein Information
predicted fused mannitol-specific PTS enzymes: IIB component/IIC component

NCBI Summary for cmtA

[More information is available at EcoCyc: EG11792].

UniProt Comments for cmtA

Function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport

By similarity.

Catalytic activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.5.

Domain: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities: Contains 1 PTS EIIB type-2 domain.Contains 1 PTS EIIC type-2 domain.

Sequence caution: The sequence CAA51229.1 differs from that shown. Reason: Contaminating sequence. Vector contamination at the C-terminus.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with cmtA recombinant protein

 Reference Product  PubMed Publications
 cmtB recombinant protein  >2 publications with cmtA and cmtB

Pathways associated with cmtA recombinant protein

 Products by Pathway  Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 PTS System, Mannitol-specific II Component Pathway antibodies  PTS System, Mannitol-specific II Component Pathway Diagram
 Phosphotransferase System (PTS) Pathway antibodies  Phosphotransferase System (PTS) Pathway Diagram
 Phosphotransferase System (PTS) Pathway antibodies  Phosphotransferase System (PTS) Pathway Diagram
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