Saccharomyces Cerevisiae ERG25 Recombinant Protein – Cat# MBS1109192

Methylsterol Monooxygenase (ERG25) Saccharomyces Cerevisiae Recombinant Protein

Name: ERG25 Recombinant Protein; Saccharomyces Cerevisiae; MyBioSource
Brand: MyBioSource
SKU: MBS1109192
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1109192

MBS1109192 is a recombinant protein targeting ERG25. Also known as Recombinant Saccharomyces cerevisiae Methylsterol monooxygenase (ERG25). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Methylsterol monooxygenase (ERG25), Recombinant Protein

Full Product Name

Recombinant Saccharomyces cerevisiae Methylsterol monooxygenase (ERG25)

Product Gene Name

ERG25 recombinant protein

Product Synonym Gene Name

ERG25; FET6

Product Synonym Names

Recombinant Methylsterol monooxygenase (ERG25); Methylsterol monooxygenase EC= 1.14.13.72; C-4 methylsterol oxidase

Sequence

MSAVFNNATL SGLVQASTYS QTLQNVAHYQ PQLNFMEKYW AAWYSYMNND VLATGLMFFL LHEFMYFFRC LPWFIIDQIP YFRRWKLQPT KIPSAKEQLY CLKSVLLSHF LVEAIPIWTF HPMCEKLGIT VEVPFPSLKT MALEIGLFFV LEDTWHYWAH RLFHYGVFYK YIHKQHHRYA APFGLSAEYA HPAETLSLGF GTVGMPILYV MYTGKLHLFT LCVWITLRLF QAVDSHSGYD FPWSLNKIMP FWAGAEHHDL HHHYFIGNYA SSFRWWDYCL DTESGPEAKA SREERMKKRA ENNAQKKTN

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

36,479 Da

Sequence Positions

1-309

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

Chromosome: VII; NC_001139.9 (610564..611493). Location: chromosome: VII

3D Structure

ModBase 3D Structure for P53045

Species

Saccharomyces cerevisiae (strain 204508 / S288c) (Baker's yeast)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ERG25 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ERG25. It may not necessarily be applicable to this product.

NCBI GI #

398365435

NCBI GeneID

852951

NCBI Accession #

NP_011574.3

UniProt Primary Accession #

P53045

UniProt Secondary Accession #

Q45U26; D6VUJ5; E9P8T3

UniProt Related Accession #

P53045

GenBank Nucleotide #

NM_001181189.3

NCBI Official Full Name

Erg25p

NCBI Official Symbol

ERG25

UniProt Protein Name

Methylsterol monooxygenase

UniProt Synonym Protein Names

C-4 methylsterol oxidase

Protein Family

Methylsterol monooxygenase

UniProt Gene Name

ERG25

UniProt Synonym Gene Names

FET6

UniProt Entry Name

MSMO_YEAST

NCBI Protein Information

Erg25p

UniProt Comments for ERG25

Function: Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol. Ref.2

Catalytic activity: 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O.4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O.3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.

Cofactor: Iron. Ref.2

Pathway: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.

Subunit structure: Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG27 and ERG28. Ref.7 Ref.10

Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein

Probable Ref.2 Ref.7 Ref.11.

Domain: The histidine box domains may contain the active site and/or be involved in metal ion binding.

Miscellaneous: Present with 77100 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the sterol desaturase family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ERG25 recombinant protein

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Zymosterol Biosynthesis Pathway antibodies	Zymosterol Biosynthesis Pathway Diagram
Zymosterol Biosynthesis Pathway antibodies	Zymosterol Biosynthesis Pathway Diagram