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Probable Diguanylate Cyclase Ydam (ydaM) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1111747

MBS1111747 is a recombinant protein targeting ydaM. Also known as Recombinant Escherichia coli Probable diguanylate cyclase YdaM (ydaM). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1150 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Probable diguanylate cyclase YdaM (ydaM), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Probable diguanylate cyclase YdaM (ydaM)
Product Gene Name
Sequence
MITHNFNTLD LLTSPVWIVS PFEEQLIYAN SAAKLLMQDL TFSQLRTGPY SVSSQKELPK YLSDLQNQHD IIEILTVQRK EEETALSCRL VLRKLTETEP VIIFEGIEAP ATLGLKASRS ANYQRKKQGF YARFFLTNSA PMLLIDPSRD GQIVDANLAA LNFYGYNHET MCQKHTWEIN MLGRRVMPIM HEISHLPGGH KPLNFVHKLA DGSTRHVQTY AGPIEIYGDK LMLCIVHDIT EQKRLEEQLE HAAHHDAMTG LLNRRQFYHI TEPGQMQHLA IAQDYSLLLI DTDRFKHIND LYGHSKGDEV LCALARTLES CARKGDLVFR WGGEEFVLLL PRTPLDTALS LAETIRVSVA KVSISGLPRF TVSIGVAHHE GNESIDELFK RVDDALYRAK NDGRNRVLAA
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
46,452 Da
Sequence Positions
1-410, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P77302
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ydaM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ydaM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
diguanylate cyclase, csgD regulator
NCBI Official Symbol
ydaM
NCBI Official Synonym Symbols
ECK1338; JW5206
UniProt Protein Name
Diguanylate cyclase DgcM
UniProt Gene Name
dgcM
UniProt Synonym Gene Names
DGCCurated
NCBI Protein Information
diguanylate cyclase, csgD regulator

NCBI Summary for ydaM

YdaM has a central PAS domain, then a C-terminal diguanylate cyclase GGDEF domain. [More information is available at EcoGene: EG13355]. YdaM controls curli biosynthesis by inducing transcription of the regulator CsgD at 28C, probably by production of the signalling molecule c-diGMP. [More information is available at EcoCyc: G6673].
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UniProt Comments for ydaM

Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two cyclic-di-GMP (c-di-GMP) control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798). DgcM stimulates activity of MlrA by direct interaction, leading to the transcription of csgD. It also catalyzes the synthesis of c-di-GMP via the condensation of 2 GTP molecules, which contributes to the c-di-GMP pool generated by module I in a positive feedback loop. Production of c-di-GMP contributes to but is not essential for MlrA activation (PubMed:17010156, PubMed:23708798).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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