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Isoleucine--Trna Ligase (ileS) Thermoanaerobacter Sp. Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1123842

MBS1123842 is a recombinant protein targeting ileS. Also known as Recombinant Thermoanaerobacter sp. Isoleucine--tRNA ligase (ileS). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$4010 / 1 mg (E-Coli) +5 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Isoleucine--tRNA ligase (ileS), Recombinant Protein
Full Product Name
Recombinant Thermoanaerobacter sp. Isoleucine--tRNA ligase (ileS)
Product Gene Name
ileS recombinant protein
Product Synonym Gene Name
ileS
Product Synonym Names
Recombinant Isoleucine--tRNA ligase (ileS); Isoleucine--tRNA ligase EC= 6.1.1.5; Isoleucyl-tRNA synthetase; IleRS
Sequence
MDYNKTLNLP RTDFPMKANL PTREPEILKR WEEMDIYHKT LEKNKGKEKY ILHDGPPYAN GDIHIGTAMN KVLKDIIVKY KTMRGYDAPY VPGWDTHGLP IEQQAIKTLG IKRHEVSPTE FRKVCRDFAF SQIEKQKAQF KRLGVRGDWD NPYLTLNPEY EAKQIEVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE YFDETSDSIY VKFRVKDDLG KFKGIVENLN NVYFVIWTTT TWTIPANLAI ALNPEFDYAL AKFGDEVYIM AKDMLDTVKK EANLSDYEIV AVFKGKDLEG MKATHPLYDR DSLIILGEHV TLEAGTGCVH TAPGHGEEDF LVGQEYGLEV LNPIDDKGYF TDKAPGYAGL YYEEANKVIK EDLKKANALV AETRITHSYP HCWRCKSPII FRATEQWFAS VEGFREEALK AIKEVNWYPS WGEERITNMV RDRRDWCISR QRVWGVPIPI FYCEKCGKPL INDDTINAVK KIFRQKGSDA WFEMSAEEIL PKGITCECGS TKFRKETDIM DVWFDSGSSH AAVLQTHPDL KWPAELYLEG SDQHRGWFQS SLLTSVATRG KAPYRNVLTH GFVVDGEGRK MSKSLGNGID PADVIKEYGA DILRLWTVSA DFTSDMRISQ EILKQMTEAY RKIRNTSKFL LSNLYDFDPD KDMLPYEELL EIDKWALFRL NRVVEELTEA FDKYEYYDFL HLVHTFCVVD MSSLYLDILK DRLYTYPATS KERRAAQTTL YIILDTLVRL IAPVLTFTSE EIWSYMKHDS QNNFESVQLA DWPQVQEKYN NPYIIEKWEK LFDIRKDISK ALEIARTDKK IGHSLEAQVD IYPSQELYDF FKGFNDLEYV FIVSKVVLHQ PEEPAPQNAY ESDDYNLKIV VTHAPGEKCE RCWMYSETVG TIKEHPTICA RCASHIEQQT QV
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
107,851 Da
Sequence Positions
1-932
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
NC_010320.1 (1900688..1903486, complement)
3D Structure
ModBase 3D Structure for B0K2W1
Species
Thermoanaerobacter sp. (strain X514)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ileS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ileS. It may not necessarily be applicable to this product.

NCBI GI #
167040517
NCBI GeneID
5877806
NCBI Accession #
YP_001663502.1
UniProt Primary Accession #
B0K2W1
UniProt Related Accession #
ZP_01454395
GenBank Nucleotide #
NC_010320.1
NCBI Official Full Name
isoleucyl-tRNA synthetase
NCBI Official Symbol
ileS
UniProt Protein Name
Isoleucine--tRNA ligase
UniProt Synonym Protein Names
Isoleucyl-tRNA synthetase
Protein Family
Isoleucine--tRNA ligase
UniProt Gene Name
ileS
UniProt Synonym Gene Names
IleRS
UniProt Entry Name
SYI_THEPX
NCBI Protein Information
isoleucyl-tRNA synthetase

UniProt Comments for ileS

Function: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)

By similarity. HAMAP-Rule MF_02002

Catalytic activity: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor: Binds 1 zinc ion per subunit

By similarity. HAMAP-Rule MF_02002

Subunit structure: Monomer

By similarity. HAMAP-Rule MF_02002

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_02002.

Domain: IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile))

By similarity. HAMAP-Rule MF_02002

Sequence similarities: Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ileS recombinant protein

 Reference Product  PubMed Publications
 ilvC recombinant protein  >43 publications with ileS and ilvC
 fusA recombinant protein  >6 publications with ileS and fusA
 alaS recombinant protein  >2 publications with ileS and alaS
 serS recombinant protein  >2 publications with ileS and serS

Pathways associated with ileS recombinant protein

 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway Diagram
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