Ralstonia Pickettii ileS Recombinant Protein – Cat# MBS1131377

Isoleucine--Trna Ligase (ileS) Ralstonia Pickettii Recombinant Protein

Name: ileS Recombinant Protein; Ralstonia Pickettii; MyBioSource
Brand: MyBioSource
SKU: MBS1131377
Price: 4095 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1131377

MBS1131377 is a recombinant protein targeting ileS. Also known as Recombinant Ralstonia pickettii Isoleucine--tRNA ligase (ileS). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. ileS More details

$4095 / 1 mg (E-Coli) +5 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Isoleucine--tRNA ligase (ileS), Recombinant Protein

Full Product Name

Recombinant Ralstonia pickettii Isoleucine--tRNA ligase (ileS)

Product Gene Name

ileS recombinant protein

Product Synonym Gene Name

Recombinant Isoleucine--tRNA ligase (ileS); Isoleucine--tRNA ligase EC= 6.1.1.5; Isoleucyl-tRNA synthetase; IleRS

Sequence

MSDSKKPTPE KSKYPVNLLD TPFPMRGDLP KREPLWVKQW QDKQLYKKIR AARKGAKKFI LHDGPPYANG DLHIGHAVNK ILKDMVIKAR GLTGLDAVYV PGWDCHGMPI EIQIEKQFGK GLPVKEVQEK ARAYATGQIA RQKADFERLG VLGDWADPYL TMNFRNEADE VRALGKILEK GYVFRGLKPV NWCFDCGSAL AEAEVEYADR TDLSIDVGFP FADIDALASA FHVGADVLKA KPGWIVIWTT TPWTIPSNQA LNLHPEIEYA LVDTSRGLLI VAKERVEACL QSWKLEGTVL ATCEGAALNG VRFHHPLAKM DAGYDRTSPV YLGDYVTIDT GTGIVHSAPA YGVEDFQSCK AHNMPDSEII NPVMGNGVYA STLPLFGGQM IWDANPKIVE VLRESGNLFD AHKYAHSYMH CWRHKTPIIY RATSQWFAGM DVQPNDGNAT LRETALAGID ATAFYPSWGK QRLHNMIANR PDWTLSRQRQ WGVPMAFFVH KETGALHPRT PELLEQVAQR IEKSGIEAWQ TLDPRELLGD EADMYEKNRD TLDVWFDSGT THWHVIRGSH AADLYDASAD LPDGRLADLY LEGSDQHRGW FHSSLLTASM LYGKPPYKGL LTHGFTVDGE GRKMSKSIGN TIAPQEIANK MGAEIIRLWV ASTDYSGELA ISDEILKRVV EGYRRIRNTL RFLLANLTDY DHAKHALPTE QWLEIDRYAV ALTDALQKEV LSHYDVYEFH PVVAKLQTFC SEDLGGFYLD VLKDRLYTTA PDSVARRSAQ NALYHITQAM LHWMAPFLSF TAEEAWQVFA HGTEHTDTIF TSTYYNAPVP QGASALLEKW AAIRNVRGEV TKQLEALRID GKIGSSLQAE VTVSAGESVH DALASLGDDL RFVLITSAAK LERAPEGGDL LITVVPSTHS KCERCWHYRA DVGHDHAHPT LCKRCTDNLF GSGEQRSAA

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

107,152 Da

Sequence Positions

1-959

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

Chromosome: 1; NC_010682.1 (2841138..2844017). Location: chromosome: 1

3D Structure

ModBase 3D Structure for B2UAQ4

Species

Ralstonia pickettii (strain 12J)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ileS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ileS. It may not necessarily be applicable to this product.

NCBI GI #

187929796

NCBI GeneID

6288807

NCBI Accession #

YP_001900283.1

UniProt Primary Accession #

B2UAQ4

UniProt Related Accession #

B2UAQ4

GenBank Nucleotide #

NC_010682.1

NCBI Official Full Name

isoleucyl-tRNA synthetase

NCBI Official Symbol

ileS

UniProt Protein Name

Isoleucine--tRNA ligase

UniProt Synonym Protein Names

Isoleucyl-tRNA synthetase

Protein Family

Isoleucine--tRNA ligase

UniProt Gene Name

ileS

UniProt Synonym Gene Names

IleRS

UniProt Entry Name

SYI_RALPJ

NCBI Protein Information

isoleucyl-tRNA synthetase

UniProt Comments for ileS

Function: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)

By similarity. HAMAP-Rule MF_02002

Catalytic activity: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor: Binds 1 zinc ion per subunit

By similarity. HAMAP-Rule MF_02002

Subunit structure: Monomer

By similarity. HAMAP-Rule MF_02002

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_02002.

Domain: IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile))

By similarity. HAMAP-Rule MF_02002

Sequence similarities: Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ileS recombinant protein

Reference Product	PubMed Publications
ilvC recombinant protein	>43 publications with ileS and ilvC
gltX recombinant protein	>2 publications with ileS and gltX
serS recombinant protein	>2 publications with ileS and serS

Pathways associated with ileS recombinant protein

Products by Pathway	Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway Diagram