GTP-Binding Nuclear Protein GSP1/CNR1 (GSP1) Recombinant Protein

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Essential for cell viability. By analogy with Ras, Ran may be activated when GTP is exchanged for bound GDP by RCC1 and inactivated when GTP is hydrolyzed by Ran upon activation by RanGAP1.

Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily.Kadowaki T., Goldfarb D., Spitz L.M., Tartakoff A.M., Ohno M.EMBO J. 12:2929-2937(1993) GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae GTP-binding proteins involved in the maintenance of nuclear organization.Belhumeur P., Lee A., Tam R., Dipaolo T., Fortin N., Clark M.W.Mol. Cell. Biol. 13:2152-2161(1993) The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.Nature 387:87-90(1997) ) Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.Genome Res. 17:536-543(2007) Protein identifications for a Saccharomyces cerevisiae protein database.Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.Electrophoresis 15:1466-1486(1994) Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1.Noguchi E., Hayashi N., Azuma Y., Seki T., Nakamura M., Nakashima N., Yanagida M., He X., Mueller U., Sazer S., Nishimoto T.EMBO J. 15:5595-5605(1996) Functional analysis of the yeast Ran exchange factor Prp20p in vivo evidence for the RanGTP gradient model.Akhtar N., Hagan H., Lopilato J.E., Corbett A.H.Mol. Genet. Genomics 265:851-864(2001) Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae.Clement M., Lavallee F., Barbes-Morin G., de Repentigny L., Belhumeur P.Mol. Genet. Genomics 266:20-27(2001) A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits.Oeffinger M., Dlakic M., Tollervey D.Genes Dev. 18:196-209(2004) Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.Mol. Cell. Proteomics 4:310-327(2005) Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery.McGuire A.T., Mangroo D.EMBO J. 26:288-300(2007)

Small volumes of GSP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1136458 is a recombinant protein targeting GSP1. Also known as Recombinant Saccharomyces cerevisiae (strain 204508 / S288c) GTP-binding nuclear protein GSP1/CNR1. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.