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Ribonuclease E (rne) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1141985

MBS1141985 is a recombinant protein targeting rne. Also known as Recombinant Escherichia coli Ribonuclease E (rne) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$385 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ribonuclease E (rne), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Ribonuclease E (rne) , partial
Product Gene Name
rne recombinant protein
Sequence
ANIYKGKITR IEPSLEAAFV DYGAERHGFL PLKEIAREYF PANYSAHGRP NIKDVLREGQ EVIVQIDKEE RGNKGAALTT FISLAGSYLV LMPNNPRAGG ISRRIEGDDR TELKEALASL ELPEGMGLIV RT
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
118,197 Da
Sequence Positions
39-170, Partial, include the S1 motif domain and interaction with RNA regions
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P21513
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of rne recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for rne. It may not necessarily be applicable to this product.

NCBI GI #
16129047
NCBI GeneID
945641
NCBI Accession #
NP_415602.1
UniProt Primary Accession #
P21513
UniProt Secondary Accession #
P77591
GenBank Nucleotide #
NC_000913.3
NCBI Official Full Name
endoribonuclease; RNA-binding protein;RNA degradosome binding protein
NCBI Official Symbol
rne
NCBI Official Synonym Symbols
ams; ECK1069; hmp1; JW1071; smbB
UniProt Protein Name
Ribonuclease E
Protein Family
Putative ribonuclease
UniProt Gene Name
rne
UniProt Synonym Gene Names
RNase E
NCBI Protein Information
endoribonuclease; RNA-binding protein;RNA degradosome binding protein

NCBI Summary for rne

The degradosome is organized with a helical cytoskeletal localization (Taghbalout, 2007). [More information is available at EcoGene: EG10859]. Ribonuclease E (RNase E) is a single-strand-specific endonuclease that is essential for viability. [More information is available at EcoCyc: EG10859].

UniProt Comments for rne

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. Can also process other RNA species, such as RNAI, a molecule that controls the replication of ColE1 plasmid, and the cell division inhibitor DicF-RNA. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic process. Required to initiate rRNA degradation during both starvation and quality control; acts after RNase PH (rph) exonucleolytically digests the 3'-end of the 16S rRNA (PubMed:27298395). Degradation of 16S rRNA leads to 23S rRNA degradation (PubMed:27298395).
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Research Articles on rne

1. This study suggests that, rather than its role in the processing of any one particular substrate, its distributed functions on all the different substrates (mRNA, rRNA, and tRNA) are responsible for the essentiality of RNase E in E. coli.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with rne recombinant protein

 Reference Product  PubMed Publications
 pnp recombinant protein  >15 publications with rne and pnp
 rnc recombinant protein  >8 publications with rne and rnc
 hfq recombinant protein  >5 publications with rne and hfq
 rhlB recombinant protein  >5 publications with rne and rhlB
 pcnB recombinant protein  >4 publications with rne and pcnB
 dnaK recombinant protein  >2 publications with rne and dnaK
 eno recombinant protein  >1 publications with rne and eno

Pathways associated with rne recombinant protein

 Products by Pathway  Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradosome Pathway antibodies  RNA Degradosome Pathway Diagram
 RNA Degradosome Pathway antibodies  RNA Degradosome Pathway Diagram
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