Nad(p) Transhydrogenase Subunit Alpha (pntA) Escherichia Coli Recombinant Protein

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.Clarke D.M., Loo T.W., Gillam S., Bragg P.D.Eur. J. Biochem. 158:647-653(1986) A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H) -induced conformational change.Ahmad S., Glavas N.A., Bragg P.D.Eur. J. Biochem. 207:733-739(1992) A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:363-377(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes.Tong R.C., Glavas N.A., Bragg P.D.Biochim. Biophys. Acta 1080:19-28(1991) Global topology analysis of the Escherichia coli inner membrane proteome.Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.Science 308:1321-1323(2005) X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli.Johansson T., Oswald C., Pedersen A., Toernroeth S., Okvist M., Karlsson B.G., Rydstroem J., Krengel U.J. Mol. Biol. 352:299-312(2005) Structure determination of a transient complex by NMR using paramagnetic distance restraints - the complex of the soluble domains of Escherichia coli transhydrogenase.Johansson T., Pedersen A., Leckner J., Karlsson B.G.Submitted (MAY-2005) to the PDB data bank

Small volumes of pntA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1145373 is a recombinant protein targeting pntA. Also known as Recombinant Escherichia coli NAD(P) transhydrogenase subunit alpha. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.