Human MBD3 Recombinant Protein – Cat# MBS1145918

Methyl-Cpg-Binding Domain Protein 3 (MBD3) Human Recombinant Protein

Name: MBD3 Recombinant Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1145918
Price: 980 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1145918

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG… More details

$980 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Methyl-CpG-binding domain protein 3 (MBD3), Recombinant Protein

Full Product Name

Recombinant Human Methyl-CpG-binding domain protein 3 (MBD3)

Product Gene Name

MBD3 recombinant protein

Sequence

MERKRWECPA LPQGWEREEV PRRSGLSAGH RDVFYYSPSG KKFRSKPQLA RYLGGSMDLS TFDFRTGKML MSKMNKSRQR VRYDSSNQVK GKPDLNTALP VRQTASIFKQ PVTKITNHPS NKVKSDPQKA VDQPRQLFWE KKLSGLNAFD IAEELVKTMD LPKGLQGVGP GCTDETLLSA IASALHTSTM PITGQLSAAV EKNPGVWLNT TQPLCKAFMV TDEDIRKQEE LVQQVRKRLE EALMADMLAH VEELARDGEA PLDKACAEDD DEEDEEEEEE EPDPDPEMEH V

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

29,014 Da

Sequence Positions

1-291, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

603573

3D Structure

ModBase 3D Structure for O95983

Species

Human

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MBD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MBD3 purchase

MBS1145918 is a recombinant protein targeting MBD3. Also known as Recombinant Human Methyl-CpG-binding domain protein 3 (MBD3). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for MBD3. It may not necessarily be applicable to this product.

NCBI GI #

527498244

NCBI GeneID

53615

NCBI Accession #

NP_001268382.1

UniProt Primary Accession #

O95983

UniProt Secondary Accession #

Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2

UniProt Related Accession #

O95983

GenBank Nucleotide #

NM_001281453.1

NCBI Official Full Name

methyl-CpG-binding domain protein 3 isoform 1

NCBI Official Synonym Full Names

methyl-CpG binding domain protein 3

NCBI Official Symbol

MBD3

UniProt Protein Name

Methyl-CpG-binding domain protein 3

UniProt Synonym Protein Names

Methyl-CpG-binding protein MBD3

Protein Family

Methyl-CpG-binding domain-containing protein

UniProt Gene Name

MBD3

NCBI Protein Information

methyl-CpG-binding domain protein 3

NCBI Summary for MBD3

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

UniProt Comments for MBD3

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

Research Articles on MBD3

1. Study have demonstrated that MBD3/ NuRD inhibits formation of liver induced cancer stem cells (iCSCs). In addition, MBD3 suppression induces c-JUN, resulting in the induction of pluripotent genes in iCSCs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MBD3 recombinant protein

Reference Product	PubMed Publications
MBD2 recombinant protein	>69 publications with MBD3 and MBD2
HDAC1 recombinant protein	>19 publications with MBD3 and HDAC1
HDAC2 recombinant protein	>9 publications with MBD3 and HDAC2
CHD4 recombinant protein	>8 publications with MBD3 and CHD4
MTA1 recombinant protein	>7 publications with MBD3 and MTA1
MTA2 recombinant protein	>7 publications with MBD3 and MTA2
RBBP7 recombinant protein	>3 publications with MBD3 and RBBP7
GATAD2A recombinant protein	>2 publications with MBD3 and GATAD2A
RBBP4 recombinant protein	>2 publications with MBD3 and RBBP4
GATAD2B recombinant protein	>2 publications with MBD3 and GATAD2B

Pathways associated with MBD3 recombinant protein

Products by Pathway	Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HDACs Deacetylate Histones Pathway antibodies	HDACs Deacetylate Histones Pathway Diagram
RNA Polymerase I Promoter Clearance Pathway antibodies	RNA Polymerase I Promoter Clearance Pathway Diagram
RNA Polymerase I Transcription Pathway antibodies	RNA Polymerase I Transcription Pathway Diagram
RNA Polymerase I Transcription Initiation Pathway antibodies	RNA Polymerase I Transcription Initiation Pathway Diagram
RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies	RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
Signaling Events Mediated By HDAC Class I Pathway antibodies	Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with MBD3 recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>8 publications with MBD3 and Disease Models, Animal
Nervous System Diseases Antibodies	>6 publications with MBD3 and Nervous System Diseases
Lung Neoplasms Antibodies	>5 publications with MBD3 and Lung Neoplasms
Carcinoma Antibodies	>5 publications with MBD3 and Carcinoma
Liver Neoplasms Antibodies	>2 publications with MBD3 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>2 publications with MBD3 and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>2 publications with MBD3 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>2 publications with MBD3 and Neoplasm Metastasis
Inflammation Antibodies	>2 publications with MBD3 and Inflammation
Autistic Disorder Antibodies	>2 publications with MBD3 and Autistic Disorder

Organs/Tissues associated with MBD3 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>14 publications with MBD3 and Brain
Blood Antibodies	>12 publications with MBD3 and Blood
Skin Antibodies	>11 publications with MBD3 and Skin
Lung Antibodies	>7 publications with MBD3 and Lung
Liver Antibodies	>7 publications with MBD3 and Liver
Embryonic Tissue Antibodies	>5 publications with MBD3 and Embryonic Tissue
Nerve Antibodies	>5 publications with MBD3 and Nerve
Testis Antibodies	>4 publications with MBD3 and Testis
Eye Antibodies	>2 publications with MBD3 and Eye
Connective Tissue Antibodies	>2 publications with MBD3 and Connective Tissue