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Alanine--Trna Ligase (alaS) Vibrio Harveyi Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1151204

MBS1151204 is a recombinant protein targeting alaS. Also known as Recombinant Vibrio harveyi Alanine--tRNA ligase (alaS). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$3815 / 1 mg (E-Coli) +4 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alanine--tRNA ligase (alaS), Recombinant Protein
Full Product Name
Recombinant Vibrio harveyi Alanine--tRNA ligase (alaS)
Product Gene Name
alaS recombinant protein
Product Synonym Gene Name
alaS
Product Synonym Names
Recombinant Alanine--tRNA ligase (alaS); Alanine--tRNA ligase EC= 6.1.1.7; Alanyl-tRNA synthetase; AlaRS
Sequence
MYMSTDEVRN AFLKFFESKG HQIVESSSLV PHNDPTLLFT NAGMNQFKDC FLGLEKRAYT RATTAQRCVR AGGKHNDLEN VGFTARHHTF FEMLGNFSFG DYFKEDAIAF AWEFLTETLK LPADRLLVTV YETDDEAFDI WNKKVGVPAD RIIRIGDKKG GKPFESDNFW QMGDTGPCGP CTEIFYDHGE HIWGGRPGTP EEDGDRFIEI WNNVFMQFNR QADGTMEPLP KPSVDTGMGI ERISAIMQGV HSNYEIDVFQ ALIKAAAEVI GYEDLSNQSL RVIADHIRSC SFLIVDGVMP SNEGRGYVLR RIIRRAVRHG NKLGAQGAFF HKLVGVLAEI MGTAGEELKR QQAVVEKVLR IEEENFGRTL ERGMAILNEA LDNISSQGTD GKVLDGETVF KLYDTYGFPA DLTNDVARER EFAIDEEGFE KAMEEQRQRA REAGNFGTDY NAAIKVDTQT EFCGYTGTKG SSSVAAMFVE GNEVESLSAG DKAIIVLGET PFYAESGGQC GDAGEIRTES GVFRVEDTQK LGNAIAHHGV MAEGVLAKGD EVATIVDAER RAAISLNHSA THLLHAALRQ LLGEHVTQKG SLVKADGLRF DFSHLEAVTA AELKEVERLV NAQIRRNHVI ETNVMDIESA KKKGAMALFG EKYDDEVRVL SMGDFSTELC GGIHASSTGD IGLFKITSEG GIAAGIRRIE AVTGEGALDA IEAQAAKYEE KLAESAQKAK SLEKEIQKLK DKMAAAESAN IMGKVQDING TKVLIAALEG ADNKNLRTMV DDIKNQVGSG VILLANVNDD KIGLIAGVTK DLIGKVKAGD LVKMVAEQVG GKGGGRPDMA QAGGTDVAAL PAAIQTVQPW LEERL
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
94,342 Da
Sequence Positions
1-865
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
Chromosome: I; NC_009783.1 (3557435..3560032, complement). Location: chromosome: I
3D Structure
ModBase 3D Structure for A7MYT5
Species
Vibrio harveyi (strain BAA-1116 / BB120)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of alaS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for alaS. It may not necessarily be applicable to this product.

NCBI GI #
156975767
NCBI GeneID
5552963
NCBI Accession #
YP_001446674.1
UniProt Primary Accession #
A7MYT5
UniProt Related Accession #
A7MYT5
GenBank Nucleotide #
NC_009783.1
NCBI Official Full Name
alanyl-tRNA synthetase
NCBI Official Symbol
alaS
UniProt Protein Name
Alanine--tRNA ligase
UniProt Synonym Protein Names
Alanyl-tRNA synthetase
Protein Family
Alanine--tRNA ligase
UniProt Gene Name
alaS
UniProt Synonym Gene Names
AlaRS
UniProt Entry Name
SYA_VIBHB
NCBI Protein Information
alanyl-tRNA synthetase

UniProt Comments for alaS

Function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain

By similarity. HAMAP-Rule MF_00036

Catalytic activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP-Rule MF_00036

Cofactor: Binds 1 zinc ion per subunit

By similarity. HAMAP-Rule MF_00036

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_00036.

Domain: Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs

By similarity. HAMAP-Rule MF_00036

Sequence similarities: Belongs to the class-II aminoacyl-tRNA synthetase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with alaS recombinant protein

 Reference Product  PubMed Publications
 recA recombinant protein  >7 publications with alaS and recA
 leuS recombinant protein  >1 publications with alaS and leuS
 lysS recombinant protein  >1 publications with alaS and lysS
 metG recombinant protein  >1 publications with alaS and metG

Pathways associated with alaS recombinant protein

 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Prokaryotes Pathway Diagram
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