Plantazolicin (pznA) Bacillus Amyloliquefaciens Recombinant Protein

Function: Peptide antibiotic inhibiting growth of Gram-positive bacteria in the dimethylated form plantazolicin A. The desmethyl form plantazolicin B has no antibiotic activity. The mode of action appears to be disruption of cell walls and lysis of cells. Inhibits B.subtilis strain HB0042, B.megaterium strain 7A1 and B.anthracis (MIC=2-4 µg/ml). Weakly inhibits Gram-positive bacteria B.brevis strain 8246, B.subtilis strain 168, B.cereus strain 14579 and strain CU1065, B.licheniformis strain 9789, M.luteus, B.sphaericus, P.granivorans and S.pyogenes (MIC=128 µg/ml). Does not inhibit B.pumilus, P.polymyxa, Arthrobacter sp., S.aureus, vancomycin-resistant E.faecalis, L.monocytogenes, methicillin-resistant S.aureus or Gram-negative bacteria E.coli strain K12, K.terrigena, Pseudomonas sp. and E.carotovora. Ref.1 Ref.3

Subcellular location: Secreted › cell wall Ref.1 Ref.3.

Post-translational modification: Maturation of thiazole and oxazole containing antibiotics involves the enzymic condensation of a Cys, Ser or Thr with the alpha-carbonyl of the preceding amino acid to form a thioether or ether bond, then dehydration to form a double bond with the alpha-amino nitrogen. Thiazoline or oxazoline ring are dehydrogenated to form thiazole or oxazole rings.2 forms exist: plantazolicin A and plantazolicin B. The structural difference between them is a dimethylation at Arg-28 in plantazolicin A. Ref.4

Mass spectrometry: Molecular mass is 1336.6 Da from positions 28 - 41. Determined by ESI. Plantazolicin A. Ref.1Molecular mass is 1308.5 Da from positions 28 - 41. Determined by ESI. Plantazolicin B. Ref.1Molecular mass is 1336.4783 Da from positions 28 - 41. Determined by MALDI. Ref.3Molecular mass is 1336.48 Da from positions 28 - 41. Determined by ESI. Plantazolicin A. Ref.4Molecular mass is 1308.44 Da from positions 28 - 41. Determined by ESI. Plantazolicin B. Ref.4